Marshall Feteri
Technical Sales Specialist
NanoString Technologies

Morphology-driven high-plex spatial analysis of tissue microenvironments

Spatial characterization of the immune contexture in the tumor microenvironment (TME) is essential for understanding the relationship between immune and tumor cells. However, it has proven difficult to perform such studies in a highly multiplexed manner using limited samples. To address this unmet need, we have developed an imaging and tissue-sampling platform designed to simultaneously analyze up to hundreds of tumor and immune proteins in distinct locations on a single FFPE tissue section. To supplement this capacity, we have recently developed an RNA detection chemistry that can profile thousands of transcripts using NGS sequencing platforms on the same FFPE sample types. Using this novel technology, called Digital Spatial Profiling (DSP), we demonstrate multiplexed protein and RNA analysis of defined regions of interest within a tumor enabling systematic interrogation of the immune status in the tumor microenvironment.

Anthony Beckhouse
Senior Sales Specialist
Illumina

Enabling High Resolution Spatial Profiling with Next Generation Sequencing

To date, most gene expression studies have been performed using various methods ranging from relative quantitative PCR, microarray, and Next Generation Sequencing (NGS) on bulk samples consisting many cells from a sample. Only recently we have seen an explosion of NGS data created from the transcriptome of single cells that make up a sample to reveal heterogeneity in cells of the same sample. Now with spatial transcriptomics we can discover gene expression profiles of specified regions of tissue samples on common histology slides giving insight into micro-environmental differences. NGS enables this high resolution, high throughput methodology through a range of gold standard sequencing instruments and well known sequencing chemistries to provide high quality data for downstream analyses. Today we will provide some guidance on experimental planning, instrument choice and information on the latest sequencing technology for your spatial transcriptomics work.