Overview
Nucleotide diversity is critical for optimal run performance and high-quality data generation. Low diversity libraries may skew software performance and data accuracy resulting in poor quality runs. We will discuss what is nucleotide diversity and why is it important. Topics will cover understanding of base imaging on Illumina sequencing platforms, strategies to optimize low diversity sequencing data, and overview of percentage base check and quality score calculation.
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Yue Xu |
Yue graduated from La Trobe University in Melbourne in 2013 with a PhD in Molecular Biology. He extended his research in programmed cell death-controlled anther fertility as a post-doctoral fellow prior to joining ARC Centre of Excellence in Plant Energy Biology to focus on mitochondrial retrograde regulation involved stress response as a Research Officer. After acquiring several years of commercial experiences in supporting academic and clinical researchers on qPCR, CE sequencing and NGS workflows, he joined Illumina as a Technical Application Scientist in August 2021. |