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Library preparation for Illumina sequencing consists of DNA fragmentation and adapter-tagging. DNA fragmentation can be achieved using enzymatic or mechanical methods. Enzymatic methods are typically more convenient but can create challenges around sensitivity to DNA quantitation, and/or require extensive optimization to achieve robustness.
Nextera Flex is a novel bead-linked-transposome technology, which addresses these shortcomings whilst retaining the convenience of enzymatic fragmentation. Nextera Flex is compatible with a wide range of DNA inputs, providing new levels of robustness while minimizing the requirement for quantitation post-library prep for an input range of 100-500ng for whole genome sequencing and 50-1000ng for targeted enrichment. In addition, the underlying technology will normalize the libraries to give a consistent yield and insert size from a range of DNA inputs. When paired with the Nextera Flex lysis kit, customers can employ a quantification-free workflow to enable normalized library creation from raw samples such as blood and saliva. This new technology offers customers a novel, easy-to-use, fast, and simple solution for library preparation.MM/DD/YYYY
The Advances in Genome Biology and Technology meeting brings together technology, software, applications, data resources and public policy. AGBT provides a forum for exchanging information about the latest advances in DNA sequencing technologies, experimental and analytical approaches for genomic studies, and their myriad applications.
Gary Schroth, Distinguished Scientist, Illumina, Inc. and Shawn Levy, Director, HudsonAlpha Institute for Biotechnology will be giving a presentation entitled: “HiSeq, NovaSeq, and iSeq: Project transitions to leverage larger scale sequencing”.Marco Island, FL MM/DD/YYYY
The ASCB annual meeting focuses on cell biology as the fundamental basis of biology
and exploring more specialized fields, such as neurobiology and stem cell biology.
The American Society for Cell Biology (ASCB) is an international community of biologists studying the cell, the fundamental unit of life. They are dedicated to advancing scientific discovery, advocating sound research policies, improving education, promoting professional development, and increasing diversity in the scientific workforce.
This event is co-sponsored by the European Molecular Biology Organization (EMBO), an organization of more than 1800 leading researchers that promotes excellence in the life sciences.Washington, DC MM/DD/YYYY
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