Nextera Flex: A Robust and Self-Normalizing Enzymatic Library Prep Technology

Nextera Flex: A Robust and Self-Normalizing Enzymatic Library Prep Technology

Andrew Slatter and Joshua Burgess, Illumina
Topic: Agriculture Genomics : Cancer Genomics : Reproductive & Genetic Health : Human Genetics & Genetic Disease : Microbiology & Infectious Disease : Sequencing : Services

Library preparation for Illumina sequencing consists of DNA fragmentation and adapter-tagging. DNA fragmentation can be achieved using enzymatic or mechanical methods. Enzymatic methods are typically more convenient but can create challenges around sensitivity to DNA quantitation, and/or require extensive optimization to achieve robustness.  

Nextera Flex is a novel bead-linked-transposome technology, which addresses these shortcomings whilst retaining the convenience of enzymatic fragmentation. Nextera Flex is compatible with a wide range of DNA inputs, providing new levels of robustness while minimizing the requirement for quantitation post-library prep for an input range of 100-500ng for whole genome sequencing and 50-1000ng for targeted enrichment. In addition, the underlying technology will normalize the libraries to give a consistent yield and insert size from a range of DNA inputs. When paired with the Nextera Flex lysis kit, customers can employ a quantification-free workflow to enable normalized library creation from raw samples such as blood and saliva. This new technology offers customers a novel, easy-to-use, fast, and simple solution for library preparation. 



Illumina Informatics, Next-Generation Sequencing and Array Seminar - UC Denver Genomics Core
University of Colorado AMC Skaggs School of Pharmacy 12850 E. Montview Blvd Aurora, Colorado 80045 USA | North America
Topic: Agriculture Genomics : Human Genetics & Genetic Disease : Microbiology & Infectious Disease
Introducing Nextera™ Flex for Enrichment
Grace DeSantis, Ph.D. Director, Product Development, Illumina, Inc.
Topic: Cancer Genomics : Human Genetics & Genetic Disease
IASLC 2019 Targeted Therapies of Lung Cancer Meeting
Santa Monica, CA | North America
Topic: Cancer Genomics