| 2:10 PM |
Emerging Single-cell Applications
Speaker:
Lei Tong
Sr. Regional Segment Manager
Illumina
Abstract:
Single-cell sequencing examines the genomes, epigenetics or transcriptomes of individual cells, and provides an unbiased high-resolution view of cell-to-cell variation. Highly sensitive single cell RNA sequencing (scRNA-Seq) methods enable assessment of the individual contribution of single cells among complex tissues by profiling their transcriptomes – providing an ideal method to understand heterogeneous subpopulation responses to treatments or environmental cues plus the cell function and heterogeneity in processes such as differentiation, proliferation, and tumorigenesis. Join us to learn about the recent advances that are enabling high throughput analysis while examining hundreds to tens of thousands of cells per experiment in a cost-effective manner.
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| 2:30 PM |
High throughput single cell multi-omics analysis with various application resolve complex cellular heterogeneity
Speaker:
박경은 (Kyoung-Eun Park)
Field Application Specialist,
MDxK
Abstract:
Single cell technologies are increasingly applied to dissect cellular composition of clinical research samples to simultaneously identify the surface and gene expression profiles of evolving immune cells, including rare populations. This webinar will showcase application of BD® AbSeq technology using 40 markers together with single cell transcriptomics utilizing the BD Rhapsody™ instrument. We will describe the workflow for analyzing these data ranging from sample preparation, cell type identification, and gene expression analysis. We will then describe a bioinformatics workflow that takes the protein and gene expression data and identifies cellular composition. Finally, we will also discuss how to integrate these data sets across different specimens and gather information on the cellular heterogeneity across these samples.
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| 2:50 PM |
Introducing NextSeq™ 1000 and NextSeq 2000
Speaker:
Kenny Choi
Sr. Sales Specialist,
Illumina
Abstract:
Innovations in next-generation sequencing (NGS) are helping the genomics community ask, and answer, increasingly complex scientific questions. Across the spectrum of oncology, microbiome research, single-cell studies, and other emerging applications, researchers need sequencing power to help fuel larger studies of more samples at greater sequencing depth and at a lower cost. These NextSeq 1000 and NextSeq 2000 offer breakthrough system design, chemistry innovations, compatibility with an expansive list of library preparation options, and on-board integrated informatics for rapid secondary analysis.
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| 3:05 PM |
NextSeq 2000 Virtual Demo
Speaker:
Kwong Shiyang
Staff Application Scientist Trainer,
Singapore Illumina Solution Center
Abstract:
The NextSeq 2000 is our latest sequencing platform which utilizes Illumina’s latest state-of-the-art sequencing technology. In this virtual session, we will highlight the key steps during a sequencing run setup. We will walk you through the simple and quick workflow on how to create the run, prepare consumable, dilute and load the sequencing library and start the run. Lastly, we will show you an example of the run and analysis data generated after the run.
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