Cancer and the Oral Microbiome: A Metagenomics Approach

Cancer and the Oral Microbiome: A Metatgenomics Approach

Scott Kelly, PhD, Professor, San Diego State University (SDSU)
Topic: Microbiology & Infectious Disease

In this study, we investigated whether salivary bacterial profiles might provide useful biomarkers for early detection of pancreatic cancer. Using high-throughput sequencing of bacterial small subunit ribosomal RNA (16S rRNA) gene, we characterized the salivary microbiota of patients with pancreatic cancer and compared them to healthy patients. A total of 146 patients were enrolled at UCSD Moores Cancer Center, where saliva and demographic data were collected from each patient. Of these, we analyzed the salivary microbiome of 108 patients: 8 had been diagnosed with pancreatic cancer, 78 with other diseases, and 22 were classified as non-diseased (healthy) controls. Bacterial 16S rRNA sequences were amplified directly from salivary DNA extractions and subjected to high-throughput sequencing (HTS). Several bacterial genera differed in abundance in patients with pancreatic cancer. This webinar will be focusing on the methods used, from gathering samples to analyzing data in the saliva. Showing a nice demonstration that bacteria abundance profiles in saliva are useful biomarkers for pancreatic cancer even though much larger patient studies are needed to verify their predictive utility.



Nextera Flex: A Robust and Self-Normalizing Enzymatic Library Prep Technology
Andrew Slatter and Joshua Burgess, Illumina
Topic: Agriculture Genomics : Cancer Genomics : Reproductive & Genetic Health : Human Genetics & Genetic Disease : Microbiology & Infectious Disease : Sequencing : Services
AGBT Annual Meeting 2019
Marco Island, FL | North America
Topic: Agriculture Genomics : Cancer Genomics : Forensic Genomics : Reproductive & Genetic Health : Human Genetics & Genetic Disease : Microbiology & Infectious Disease : HLA Typing
ASM Microbe 2019
San Francisco, CA | North America
Topic: Microbiology & Infectious Disease