MiSeq™: How to start a run

6 April 2022

This video demonstrates the steps to set-up a run on the Illumina MiSeq system upgraded to Microsoft Windows 10 and the MiSeq Control Software V4. We will set-up the run using Local Run manager (LRM) V3 and the FASTQ generation module. Check the link below do download FASTQ generation and additional modules for LRM:https://customprotocolselector.net/selectors/LRM-module-selector/Content/Source/FrontPages/LRM-module-selector.htm Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. MLR ID:M-GL-00731 *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM 00:00 Intro 00:34 Consumables 01:06 Set up a run in Local Run Manager 03:13 Load Sample Libraries 04:29 How to start a run 05:34 Load the Flow Cell 07:22 Load PR2 and Check the Waste Bottle 09:43 Load the Reagent Cartridge 10:43 Start the Run

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