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Illumina Microbial Amplicon Prep—Influenza A/B

A streamlined whole-genome NGS library prep kit for high-impact research and public health surveillance of influenza A and B viruses.

Overview

Illumina Microbial Amplicon Prep—Influenza A/B is an amplicon-based library preparation kit built on the same chemistry as the COVIDSeq assay. Library preparation is fast and easy, generating libraries compatible with nearly all Illumina sequencing systems. This kit enables whole-genome sequencing of influenza A/B viruses for public health surveillance and influenza virus research.

Expand your public health surveillance and influenza virus research capabilities

Get sequencing results with exceptional data quality and accuracy at any scale with fast turnaround times.

Key features

  • Multiplexed, automation-friendly RT-PCR and tagmentation-based workflow

  • Simplified data analysis and reporting with the DRAGEN Targeted Microbial App on BaseSpace Sequence Hub 

  • Excellent performance with RNA from a range of sample types, including cultures and nasal swabs

This kit provides exceptional coverage across influenza A/B virus genomes, addressing sequencing challenges related to high genomic variability. In addition, achieve rapid, unbiased, and accurate influenza virus sequencing and characterization to support high-impact genomic surveillance and research efforts.

Specifications

Automation capability Liquid Handling Robots
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System
Method Targeted RNA Sequencing, Amplicon Sequencing
Nucleic acid type RNA
Species category Virus
Strand specificity Non-Stranded
Technology Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels)

Applications

Example workflow

1
Prep

Illumina Microbial Amplicon Prep—Influenza A/B

2
Sequence
3
Analyze

Related applications and methods

Public health surveillance

Genomics can advance public health and wellness by detecting and characterizing new, emerging, and circulating pathogens.

Genomic surveillance

NGS supports effective genomic surveillance strategies through identification of novel strains of coronavirus and other emerging infectious disease agents.

Microbial genomics

Next-generation sequencing (NGS) is changing microbial genomics. Use NGS to discover novel microbes, monitor outbreaks, analyze food sources, and more.

Documentation

Product literature
Support documentation

Compare

Illumina Microbial Amplicon Prep—Influenza A/B Illumina Microbial Amplicon Prep Respiratory Pathogen ID/AMR Enrichment Panel Kit Illumina Respiratory Virus Enrichment Kit
Automation capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System MiSeq System, NextSeq 550 System, MiniSeq System MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiniSeq System
Method Targeted RNA Sequencing, Amplicon Sequencing Amplicon Sequencing Target Enrichment, Targeted RNA Sequencing, Targeted DNA Sequencing Target Enrichment, Targeted RNA Sequencing, Targeted DNA Sequencing
Nucleic acid type RNA DNA, RNA DNA, RNA DNA, RNA
Species category Virus Other, Yeast, Virus, Bacteria Fungal, Virus, Bacteria Human, Virus
Strand specificity Non-Stranded
Technology Sequencing Sequencing Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs) Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels)

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Illumina® Microbial Amplicon Prep—Influenza A/B (48 samples)

20106305

Complete amplicon-prep based library prep kit for whole genome sequencing of Influenza A and B virus strains. Amplicon sequencing technology is based on well established COVIDSeq but optimized for Influenza A/B viral sequencing. Each kit provides enough reagents for 48 samples and includes library prep reagents, an influenza A/B targeting primer pool, and an index plate with 48 unique dual indexes.

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