Multiomics

What is multiomics?

Multiomics (multiple omics) provides an integrated approach to power discovery across multiple levels of biology. By combining data from genomics, transcriptomics, epigenetics, and proteomics, researchers can achieve a more comprehensive understanding of molecular changes contributing to normal development, cellular response, and disease.

Multiomics can also combine separate omic data from past experiments, known as in-silico multiomics, to efficiently analyze novel biological relationships. Regardless of the method chosen, Illumina offers solutions powered by NGS to enable your next multiomics studies and analyses.

Multiomics is revolutionizing research

Learn why these research leaders decided to use multiomics in their work.

Multiomics multiplies your discovery power

See how you can use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single omics approaches.

Download in-depth eBook

Expand your research across multiple omes

Genomics

Focus on the structure, function, evolution, mapping, and editing of information coded within an organism’s DNA.

Transcriptomics

Study the complete set of RNA transcripts produced by the genome.

Proteomics

Evaluate protein expression for a better understanding of cellular function and prediction of therapeutic responses.

Epigenomics

Examine heritable changes in gene expression activity caused by factors other than DNA changes.

Leveraging bulk to targeted methodologies for more powerful discoveries

Perform multiomic experiments using NGS-based solutions to find novel links between biological entities, identify biomarkers, profile mutations and other phenotypes, and improve discovery of new therapies. Illumina offers comprehensive solutions across biological resolutions from broad bulk analysis to targeted single cells. For more details, visit our multiomic methods comparison guide.

Comprehensively analyze bulk cell populations or tissue sections

Explore how pooling cells or tissue populations can be used to analyze protein and transcriptional activity within a single workflow using bulk epitope and nucleic acid sequencing (BEN-Seq).

Achieve high resolution data with single-cell analysis

Measure gene regulatory networks and gain insights into cell heterogeneity by combining transcriptomics and epigenomics in a singular molecular readout using ATAC-Seq found in this technical note.

See how cellular indexing of transcriptomes and epitopes sequencing (CITE-Seq) can provide proteomic and transcriptomic data in a single run powered by NGS.

Spatially map transcriptional activity in tissues

Learn key benefits of spatial transcriptomics, which localizes gene expression within intact tissues for a better understanding of structure and activity.

Streamline your multiomic workflows

Multiomic workflows generally start with nucleic acid isolation using a kit of your choice followed by creation of libraries for sequencing. Results are analyzed using bioinformatics software solutions and are dependent on the sample type, instrument, analytical method, and desired insight. A general overview is provided along with featured products to help visualize multiomic workflows. Explore the sample workflows and solution options for bulk sequencing, single-cell, and spatial sequencing methods.

Prepare Libraries

DNA Library Preparation

Explore flexible and efficient DNA library prep for a broad range of applications. Illumina DNA PCR Free Prep and Illumina DNA Prep with Enrichment offer a high-performing, fast, and integrated workflow for sensitive applications.

RNA Library Preparation

Rapid and scalable solutions to analyze transcriptomes. Illumina Total RNA Prep with Ribo-Zero Plus provides exceptional performance for the analysis of coding and multiple forms of noncoding RNA. Illumina Stranded mRNA Prep offers a streamlined RNA-Seq solution for clear and comprehensive analysis across the transcriptome.

Sequence

Production-Scale Sequencers

Access multiple omics on a single instrument. Deep and broad coverage through advanced applications for a comprehensive view of omic data with the NovaSeq X Series and NovaSeq 6000 Sequencing System.

Benchtop Sequencers

Flexible, affordable, and scalable to help both new and experienced users achieve fast turnaround times and reduced operating costs with NextSeq 1000 and NextSeq 2000 Sequencing Systems.

Analyze Data

Illumina Connected Analytics

A secure genomic data platform to operationalize informatics and drive scientific insights.

DRAGEN Analysis

Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing data.

Correlation Engine

Correlation Engine is an interactive knowledge base where users can put their private multiomic data into biological context with highly curated public multiomic data.

Simplify analysis for multiomic interpretation and insight

Following sequencing, data analysis provides the key step to allow biological interpretation of samples. In general, the multiomics data analysis pipeline consists of three phases, with available software for each step. The approach used will depend on the research objective and the omes you are studying.

Primary Analysis

Also referred to as base calling, primary analysis coverts data into base sequences (A, T, C, or G) as a raw data file in binary base call (BCL) format and is performed automatically on Illumina sequencers.

Secondary Analysis

The BCL sequence file format requires conversion to FASTQ format for use with Illumina, user-developed, or third-party secondary analysis tools. Illumina DRAGEN Secondary Analysis features tools for every step of most secondary analysis pipelines.

Tertiary Analysis

Correlation Engine is an interactive knowledge base where users can put their private multiomic data into biological context with highly curated public multiomic data.

Multiomics offers a holistic view of biology

Integrating methods for deeper insights and discoveries

What is multiomics?

Multiomics is revolutionizing research

Multiomics multiplies your discovery power

Expand your research across multiple omes

Genomics

Transcriptomics

Proteomics

Epigenomics

Leveraging bulk to targeted methodologies for more powerful discoveries

Comprehensively analyze bulk cell populations or tissue sections

Achieve high resolution data with single-cell analysis

Spatially map transcriptional activity in tissues

Streamline your multiomic workflows

Prepare Libraries

DNA Library Preparation

RNA Library Preparation

Sequence

Production-Scale Sequencers

Benchtop Sequencers

Analyze Data

Illumina Connected Analytics

DRAGEN Analysis

Correlation Engine

Simplify analysis for multiomic interpretation and insight

Primary Analysis

Secondary Analysis

Tertiary Analysis

Frequently Purchased Together

Additional Resources

Advancing cancer research with multiomics

Success with single-cell multiomics

Multiomics with NGS-based proteomics

For every lab, everywhere

NextSeq 1000 and NextSeq 2000 Reagents

NGS Workflow Finder

DRAGEN v4.3 now available on-premises and on-cloud

Illumina Proactive Instrument Performance Service

Do more, faster than ever

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

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For every lab, everywhere

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For every lab, everywhere

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For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

Multiomics

Multiomics offers a holistic view of biology

Integrating methods for deeper insights and discoveries

What is multiomics?

Multiomics is revolutionizing research

Multiomics multiplies your discovery power

Expand your research across multiple omes

Leveraging bulk to targeted methodologies for more powerful discoveries

Comprehensively analyze bulk cell populations or tissue sections

Achieve high resolution data with single-cell analysis

Spatially map transcriptional activity in tissues

Streamline your multiomic workflows

Prepare Libraries

Sequence

Production-Scale Sequencers

Benchtop Sequencers

Analyze Data