SurePlex DNA Amplification System

Used to generate a DNA template from single cells. Ideal for use with PGS research applications.

~3 hours

Assay time

~1 hr

Hands-on time

Single cell or multi-cell samples

Input quantity

See full details in the specifications table

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Overview
Applications
Documentation

Overview

Whole-genome amplification technology can be used to increase the quantity of DNA in a single cell or a few cells. This amplification ensures sufficient amounts of DNA for next-generation sequencing of preimplantation genetic screening (PGS) samples.

The SurePlex DNA Amplification System reproducibly amplifies total DNA from single cells or a few cells of an embryo biopsy. It follows an easy-to-use single tube protocol to produce 2-5 μg of amplified DNA in 2 hours. Following SurePlex DNA amplification, samples are ready for use in research applications such as VeriSeq PGS (available from Vitrolife).

Specifications

Assay time	~3 hours
Hands-on time	~1 hr
Input quantity	Single cell or multi-cell samples
Method	Targeted DNA sequencing
Nucleic acid type	DNA
Specialized sample types	Single cells
Species category	Human
Technology	Sequencing
Variant class	Chromosomal abnormalities, Structural variants, Copy number variants (CNVs)

Applications

Related applications and methods

Preimplantation genetic screening research

Preimplantation genetic screening (PGS), also known as PGT-A, determines the chromosomal status of an embryo by screening all 23 chromosome pairs.

Karyomapping research

Karyomapping techniques can assess the likelihood of an embryo carrying a gene mutation that may be linked to an inherited genetic disorder.

Cytogenomics

Together, next-generation sequencing and arrays enable cytogenomic analysis of chromosome aberrations for constitutional and cancer research.

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

Infinium Global Clinical Research Array Documentation

SurePlex DNA Amplification System Support Documentation

All SurePlex DNA Amplification System Support

SurePlex DNA Amplification System (50 reactions)

PR-40-415101-00

Buffers and reagents for 50 reactions to amplify total DNA from single cells. Intended for use with 24sure assay or VeriSeq PGS applications.

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SurePlex DNA Amplification System

~3 hours

~1 hr

Single cell or multi-cell samples

Overview

Specifications

Applications

Related applications and methods

Documentation

Product literature

Support documentation

Selection summary

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For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

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For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

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For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

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For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

SurePlex DNA Amplification System

~3 hours

~1 hr

Single cell or multi-cell samples

Overview

Specifications

Applications

Related applications and methods

Documentation

Product literature

Support documentation

Selection summary

NovaSeq X Series ordering

MiSeq Reagent Kit v3

TruSight Oncology 500

Illumina DNA Prep

MiSeq Reagent Kit v3