NovaSeq X Series ordering
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Used to generate a DNA template from single cells. Ideal for use with PGS research applications.
Assay time
Hands-on time
Input quantity
Whole-genome amplification technology can be used to increase the quantity of DNA in a single cell or a few cells. This amplification ensures sufficient amounts of DNA for next-generation sequencing of preimplantation genetic screening (PGS) samples.
The SurePlex DNA Amplification System reproducibly amplifies total DNA from single cells or a few cells of an embryo biopsy. It follows an easy-to-use single tube protocol to produce 2-5 μg of amplified DNA in 2 hours. Following SurePlex DNA amplification, samples are ready for use in research applications such as VeriSeq PGS (available from Vitrolife).
Assay time | ~3 hours |
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Hands-on time | ~1 hr |
Input quantity | Single cell or multi-cell samples |
Method | Targeted DNA sequencing |
Nucleic acid type | DNA |
Specialized sample types | Single cells |
Species category | Human |
Technology | Sequencing |
Variant class | Chromosomal abnormalities, Structural variants, Copy number variants (CNVs) |
Preimplantation genetic screening research
Preimplantation genetic screening (PGS), also known as PGT-A, determines the chromosomal status of an embryo by screening all 23 chromosome pairs.
Karyomapping techniques can assess the likelihood of an embryo carrying a gene mutation that may be linked to an inherited genetic disorder.
Together, next-generation sequencing and arrays enable cytogenomic analysis of chromosome aberrations for constitutional and cancer research.
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SurePlex DNA Amplification System (50 reactions)
PR-40-415101-00
Buffers and reagents for 50 reactions to amplify total DNA from single cells. Intended for use with 24sure assay or VeriSeq PGS applications.
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