Now is the time to upgrade for more discovery power. Support and reagent availability for HiSeq 1000–3000 Systems ends February 28, 2023. Contact us to see if you qualify for a trade-in or use our Sequencing Platform Comparison Tool to find the best instrument for your needs.
The HiSeq Rapid Duo cBot Sample Loading Kit allows maximum indexing freedom in rapid run mode by enabling individually addressable lanes. This kit is an optional accessory for HiSeq Rapid Cluster kits (which require a cBot instrument), and is designed for use in the rapid run workflow of HiSeq 2500 and 1500 sequencers.
The HiSeq Rapid Duo cBot Sample Loading Kit allows each rapid flow cell lane to be loaded with a unique sample template pool. The duo kit accomplishes individual lane loading by allowing a cBot instrument to be used for the template hybridization and first extension steps of the cluster generation process. To use the cBot to load 2 libraries onto a rapid flow cell, 1 HiSeq Rapid Duo cBot Sample Loading Kit is required. Each kit contains sufficient reagents for loading 1 flow cell.
A specifically designed duo manifold directs each of the 2 samples into the 2 separate rapid flow cell lanes. The cBot portion takes about an hour, after which the flow cell is moved to the HiSeq 2500 or 1500 for further cluster generation steps using a HiSeq Rapid Cluster Kit.
The HiSeq Rapid Duo cBot Sample Loading Kit allows you to use up to 96 unique indexes per lane on the rapid flow cell. This doubles the indexing capability achieved with the on-board cluster generation process alone. Using 2 HiSeq Rapid Duo cBot Sample Loading Kits for 2 rapid flow cells enables up to 384 unique indexes per run on a HiSeq 2500 in rapid run mode.
For many applications, it's necessary to keep samples physically distinct rather than simply indexing to distinguish one sample from another. For these applications, the HiSeq Rapid Duo cBot Sample Loading Kit offers an ideal solution.
This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.
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The TruSeq Rapid Duo cBot Sample Loading Kit has been discontinued. The HiSeq Rapid Duo cBot Sample Loading Kit is the recommended replacement. Illumina remains committed to providing you with high-quality support and service.