Now is the time to upgrade for more discovery power. Support and reagent availability for HiSeq 1000–3000 Systems ends February 28, 2023. Contact us to see if you qualify for a trade-in or use our Sequencing Platform Comparison Tool to find the best instrument for your needs.

HiSeq Rapid SBS Kit v2

HiSeq Rapid SBS Kits v2 are designed for the rapid run mode of HiSeq 2500 and 1500 sequencers. Read More...
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HiSeq Rapid SBS Kit v2 (50 cycles)



HiSeq Rapid SBS Kit v2 (200 cycles)



HiSeq Rapid SBS Kit v2 (500 cycles)


Illumina Advantage Products

TG HiSeq® Rapid SBS Kit v2 (200 Cycle)



TG HiSeq® Rapid SBS Kit v2 (50 Cycle)



Product Highlights

HiSeq Rapid v2 SBS Kits enable the longest reads on HiSeq instruments, with reads up to 2x250 bp.  The new kits generate up to 300 Gb of data per run. HiSeq Rapid SBS Kits v2 are the ideal choice for sequencing on HiSeq systems. These kits:

  • Maximize the data quality of your runs
  • Improve turnaround time and batching efficiency
  • Provide multiple kit configurations
  • Allow you to set up once and walk away

Maximize data quality – HiSeq Rapid SBS Kits v2 are formulated to provide the highest data quality with reads up to 2x250 bp. Building on the well-established SBS chemistry, HiSeq Rapid SBS Kits integrate innovative features that increase Q-scores, decrease error rates and deliver unparalleled data quality.

Turn samples faster – HiSeq Rapid SBS Kits v2 support the rapid run workflow for the HiSeq 2500 and 1500, and allow you to turn urgent samples around fast. The lower overall data throughput of rapid run mode also simplifies batching requirements and allows optimal indexing of small genomes and other applications with reduced output needs.

Kit configurations – HiSeq Rapid SBS Kits v2 are available in three configurations: 500 cycle, 200 cycle, and 50 cycle. All HiSeq Rapid SBS v2 Kits provide sufficient reagents to support single or dual indexing workflows. Now with the ability to read up to 2 × 250 base pairs.

True walk-away workflow – Use on-board cluster generation and local sample loading on the HiSeq 2500 and 1500 for a high-throughput, walk-away workflow. All required reagents for cluster generation, paired end turn, and indexing can be loaded onto the instrument along with your sample template. Then, simply start the run and walk away. Stream runs to BaseSpace for even greater flexibility.

This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Frequently Purchased Together

If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.


Best Practices for Storing Sequencing Reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

View Bulletin
Best Practices for Storing Sequencing Reagents

Product Literature

Literature is not currently available for this product.

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

All HiSeq Rapid SBS Kit v2  Support  

The TruSeq Rapid SBS Kits (200 Cycle and 50 Cycle) have been discontinued. HiSeq Rapid SBS v2 Kits are the recommended replacements. Illumina remains committed to providing you with high-quality support and service.

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