The AmpliSeq for Illumina Comprehensive Cancer Panel provides a targeted resequencing solution for analyzing somatic mutations across 409 genes with known associations to cancer. Key features include:
The Comprehensive Cancer Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel detects variants within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate malignancies. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Instrument | Recommended Number of Samples | Read Length |
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NextSeq 550 System | Samples per run: mid output: 4, high output: 12 (assumes minimum coverage of 500×) | 2 × 150 bp |
AmpliSeq for Illumina Comprehensive Cancer Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | |
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Assay Time | 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
Cancer Type | Pan-Cancer, Solid Tumor | Pan-Cancer, Solid Tumor |
Content Specifications | Full exon coverage of 409 genes associated with cancer. | Hotspot regions of 50 genes with known associations to cancer |
Description | Somatic analysis research across pan-cancer genes. | Somatic analysis research into hotspot regions of 50 cancer-related genes. |
Hands-On Time | < 1.5 hours | < 1.5 hours |
Input Quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) |
Method | Amplicon Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing |
Multiplexing | 96 dual index combinations | 96 dual index combinations |
Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue |
Species Category | Human | Human |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
AmpliSeq for Illumina Comprehensive Cancer Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Comprehensive Cancer Panel Gene List
product_file | EXCEL < 1 MB
NextSeq 550 amplicon sequencing solution
Application Note | PDF < 1 MB
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Reference Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Documentation
AmpliSeq for Illumina Comprehensive Cancer Panel Checklist Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Comprehensive Cancer Panel Support