Order the NovaSeq X Series
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Targeted panel to investigate 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers.
Assay time
Hands-on time
Input quantity
The Myeloid Panel enables concurrent analysis of both DNA and RNA from blood and bone marrow samples in a single assay to study biomarkers associated with hematologic malignancies. The panel covers relevant targets for these major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). Key features include:
The Myeloid Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.
The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Assay time | 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
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Cancer type | Hematologic |
Content specifications | Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) |
Description | Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. |
Hands-on time | < 1.5 hr |
Input quantity |
20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool) |
Instruments | MiSeq System, MiniSeq System |
Method | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing |
Multiplexing | Up to 96-plex |
Nucleic acid type | DNA, RNA |
Specialized sample types | Blood, Not FFPE-compatible, Bone marrow |
Species category | Human |
Technology | Sequencing |
Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) |
The AmpliSeq for Illumina Myeloid Panel workflow requires the Myeloid Panel oligos, one of the AmpliSeq Library PLUS kits, and at least one AmpliSeq index kit. cDNA synthesis is required for converting total RNA to cDNA.
AmpliSeq for Illumina Myeloid Panel
Instrument | Recommended number of samples | Read length |
---|---|---|
MiniSeq System | Samples per run (mid output): 4 DNA, 32 RNA, 3 combined samples (based on >95% at 500x minimum coverage) |
2 x 151 bp |
MiSeq System | Samples per run with v2 reagents: 7 DNA, 60 RNA, 6 combined samples |
2 x 151 bp |
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Illumina NGS and microarray technologies for cancer research are helping drive the revolution in cancer genomics.
AmpliSeq for Illumina Myeloid Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | TruSight Myeloid Sequencing Panel | |||
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Assay time | 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 8 hours | ||
Cancer type | Hematologic | Pan-cancer, Solid tumor | Hematologic | ||
Content specifications | Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) | Hotspot regions of 50 genes with known associations to cancer | Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots. | ||
Description | Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. | For somatic mutation detection studies in myeloid malignancies. | ||
Hands-on time | < 1.5 hr | < 1.5 hr | 3 hr | ||
Input quantity |
20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool) |
1–100 ng (10 ng recommended per pool) | 50 ng | ||
Instruments | MiSeq System, MiniSeq System | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System | ||
Method | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Amplicon sequencing | ||
Multiplexing | Up to 96-plex | 96 dual index combinations | Up to 96-plex | ||
Nucleic acid type | DNA, RNA | DNA | DNA | ||
Specialized sample types | Blood, Not FFPE-compatible, Bone marrow | FFPE tissue | Not FFPE-compatible, Not FFPE-compatible | ||
Species category | Human | Human | Human | ||
Technology | Sequencing | Sequencing | Sequencing | ||
Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) | Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels) | Somatic variants, Insertions-deletions (indels) |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
High coverage of CEBPA gene
DNA evaluated with AmpliSeq for Illumina Myeloid Panel shows high read depth coverage across the entire transcript of the gene.
AmpliSeq™ for Illumina® Myeloid Panel
20024478
Fixed panel includes 74 genes associated with hematologic malignancies. Kit contains 24 reactions (24 DNA libraries and 24 RNA libraries). Requires 48 reactions of LP PLUS. Library PLUS, indexes and cDNA synthesis sold separately.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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