AmpliSeq for Illumina TCR beta-SR Panel

FFPE-compatible panel for measuring T cell diversity and clonal expansion in tumor samples by sequencing T cell receptor beta chain rearrangements.Read More...
Select Product(s)
What Products do I need?
Panel

AmpliSeq™ for Illumina® TCR beta-SR Panel

20031675

Price
 
 

Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

Price
 
 

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

Price
 
 

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

Price
 
 

Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

Price
 
 

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

Price
 
 

AmpliSeq™ CD Indexes Set B for Illumina®

20019106

Price
 
 

AmpliSeq™ CD Indexes Set C for Illumina®

20019107

Price
 
 

AmpliSeq™ CD Indexes Set D for Illumina®

20019167

Price
 
 

AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

Price
 
 
Accessory Products
What accessories do I need?

Ampliseq™ cDNA Synthesis for Illumina®

20022654

Price
 
 

AmpliSeq™ Library Equalizer for Illumina®

20019171

Price
 
 

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

Price
 
 

Product Highlights

AmpliSeq for Illumina TCR beta-SR (Short Read) Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. It is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. You can use a single pool of multiplex PCR primers, library reagents, and sample barcodes to generate libraries from DNA/RNA extracted from FFPE, whole blood, fresh-frozen tissue, or fluorescence activated cell sorting (FACS)-sorted cells.

Key features include:

  • High-Value Content
    Obtain comprehensive coverage of TCR beta chain sequences
  • Simple, Streamlined Workflow
    Prepare sequencing-ready libraries in a single day from FFPE, whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells
  • Low Input Requirement
    Generate high-quality sequencing libraries from as little as 10 ng of input RNA

The AmpliSeq for Illumina TCR beta-SR Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. It enables characterization of the hypervariable complementary determining region 3 (CDR3) of the TCR beta chain. 

Learn More About AmpliSeq for Illumina

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run will depend on sample type. 2 × 151 bp
MiSeq System Samples per run will depend on sample type. 2 × 151 bp
NextSeq System Samples per run will depend on sample type. 2 × 151 bp

Product Comparison

AmpliSeq for Illumina TCR beta-SR Panel AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel
Assay Time 5.5-7.5 hours 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Content Specifications Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3. RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons)
Hands-On Time <1.5 hours < 1.5 hours
Input Quantity 1 ng 10–1,000 ng
Method Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted RNA Sequencing
Nucleic Acid Type RNA, DNA RNA
Specialized Sample Types Blood, FACS Cells, FFPE Tissue, Fresh/Frozen Tissue Blood, FACS Cells, Fresh/Frozen Tissue
Species Category Human Human
System Compatibility MiniSeq , NextSeq 550 MiniSeq , MiSeq , MiSeq, NextSeq 550

Method-Specific Workflow Example

 

Supporting Data and Figures

 

Related Products

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel

Targeted RNA research panel to investigate T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements.


AmpliSeq for Illumina Custom RNA Panel

Targeted, custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.


AmpliSeq for Illumina Custom DNA Panel

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.