AmpliSeq for Illumina Transcriptome Human Gene Expression Panel

Targeted panel that measures expression levels of >20,000 human RefSeq genes. Read More...
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Panel

AmpliSeq™ Transcriptome Human Gene Expression Panel for Illumina®

20019170

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Accessory Products

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay. Key features include:

Fast Gene Expression Profiling

Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.

Low-Quality, Low-Quantity Sample Input

Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.

DNA-to-Analysis Solution

Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.

The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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Specifications

Product Comparison

AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome TruSeq Stranded Total RNA TruSeq Stranded Total RNA with Ribo-Zero Globin
Content Specifications > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA
Description Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.
Input Quantity 1-100 ng RNA (10 ng recommended) 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. 0.1 – 1 ug high-quality purified total RNA from blood
Method Exome Sequencing, mRNA Sequencing, Targeted RNA Sequencing Metatranscriptome Sequencing, Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing
Specialized Sample Types Blood, FFPE Tissue FFPE Tissue Blood
Species Category Human Bacteria, Human, Mouse, Rat Human, Mouse, Rat Human, Mouse, Rat

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