Performance Parameters and Requirements
| Maximum Read Length | Up to 2 × 300 bp (Refer to package insert for assay-dependent specifications) |
|---|---|
| Output (2 x 150 bp run) | ≥ 5 Gb |
| Reads passing filters (2 x 150 bp run) | ≥ 15 million |
| Accuracy-Germline* | >99.9% Overall Percent Agreement to Reference Results |
| Accuracy-Somatic* | 100% Overall Percent Agreement to Reference Results |
| Reproducibility-Germline** | 99.88% Overall Percent Agreement to Reference Results |
| Reproducibility-Somatic*** | 99.6% Percent Expected Call for mutant samples |
| Q30 score (at read length of 2 × 150 bp) | ≥ 80% |
| Power Requirements | 90–264V AC at 50/60Hz, 10A, 400W |
| RFID Radio Frequency | 13.56 MHz |
| RFID Power | 100 mW |
| Samples per Run | Depending on assay |
| LED | 520, 660 nm |
*Results based on a representative TruSeq Amplicon-based assay designed to query a variety of genes covering 12,588 bases across 23 different chromosomes using 150 amplicons
**Results based on Cystic Fibrosis (CF-139) Assay
***Results based on a representative 2-gene TruSeq Amplicon-based assay
Storage and Handling
| Temperature | Transportation and Storage: -10°C to 40°C (14°F to 104°F) Operating Conditions: 19°C to 25°C (66°F to 77°F) |
|---|---|
| Humidity | Transportation and Storage: Non-condensing humidity Operating Conditions: 30–75% relative humidity (non-condensing) |
Dimensions
| W×D×H | 68.6 cm × 56.5 cm × 52.3 cm (27.0 in × 22.2 in × 20.6 in) |
|---|---|
| Weight | 54.5 kg (120 lbs) |
| Crated Weight | 90.9 kg (200 lbs) |
Instrument Control Computer
| Base Unit | Intel Core i7-7700 2.9 GHz CPU |
|---|---|
| Memory | 16 GB RAM |
| Hard Drive | 2 × 1 TB |
| Operating System | Windows 10 |
Specifications shown here are for IVD use. View research use only specs, applicable only when running MiSeqDx in Research mode.
MiSeqDx System Specification Sheet
Designed specifically for the clinical laboratory environment, the MiSeqDx instrument offers a small, approximately 4 square feet (0.3 square meters) footprint, an easy to follow workflow, and data output tailored to the needs of clinical labs.
View Specification SheetKey Technology
Sequencing by Synthesis (SBS) Technology
The MiSeqDx System harnesses proven Illumina SBS technology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
Learn MorePaired-End Sequencing Technology
Paired-end sequencing enables both ends of the DNA fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads over repetitive regions more precisely. This results in much better alignment of the reads, especially across difficult-to-sequence, repetitive regions of the genome.
Learn More