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NextSeq 1000 & NextSeq 2000 Specifications

NextSeq 1000 and NextSeq 2000
Specifications

Instrument configuration
  • Self-contained, dry instrument with integrated DRAGEN Bio-IT FPGA secondary analysis
    • Base Unit: 2U Microserver located inside the instrument
    • Memory: 288 GB
    • Hard Drive: 3.8 TB SSD
    • Operating System: Linux CentOS 7.6
    • Temperature: 15°C-30°C
    • Humidity: 20%-80% relative humidity, non-condensing
    • Altitude: 0 meters-2000 meters
    • For Indoor Use Only
    • Wavelengths: 449 nm, 523 nm, 820nm
    • Safety: Class 1 Laser Product
    • W×D×H: 60 cm × 65 cm × 60 cm
    • Weight: 141 kg
    • Crated W×D×H: 92 cm × 120 cm × 118 cm
    • Crated Weight: 232 kg
    • Instrument Input Voltage: 100Vac to 240Vac
    • Instrument Input Frequency: 50/60Hz
    • 200 Mb/s/instrument for internal network uploads
    • 200 Mb/s/instrument for BaseSpace Sequence Hub uploads
    • 5 Mb/s/instrument for Instrument Operational Data uploads
    • NRTL certified IEC
    • 61010-1 CE marked
    • FCC/IC approved
  • Instrument control computer
    Operating environment
    Laser
    Dimensions
    Crated Dimensions
    Power requirements
    Bandwidth for network connection
    Product safety and compliance
    Download Specifications PDF
nextseq 2000 system drawing

NextSeq 1000 and NextSeq 2000
Cartridge

Easy-to-use cartridge-based platform

The NextSeq 1000 and NextSeq 2000 Sequencing Systems take advantage of an integrated cartridge that includes reagents, fluidics, and the waste holder, simplifying library loading and instrument use. Simply thaw the reagent cartridge, insert the flow cell into the cartridge, load the library into the cartridge, and insert the assembled cartridge into the instrument.

NextSeq 1000 and NextSeq 2000
Flow Cell

Increased output

To take full advantage of higher density flow cells, the NextSeq 1000 and NextSeq 2000 Sequencing Systems feature a novel super resolution optics system that yields highly accurate imaging data with greater resolution and higher sensitivity than traditional benchtop systems. This miniaturization provides scalability for a variety of output quantities while maintaining the same high standards of data quality enjoyed by NextSeq 550 System users today.

NextSeq 2000 Cartidge
NextSeq 2000 Flow Cell

NextSeq 1000 and NextSeq 2000
Flow Cell

Increased output

To take full advantage of higher density flow cells, the NextSeq 1000 and NextSeq 2000 Sequencing Systems feature a novel super resolution optics system that yields highly accurate imaging data with greater resolution and higher sensitivity than traditional benchtop systems. This miniaturization provides scalability for a variety of output quantities while maintaining the same high standards of data quality enjoyed by NextSeq 550 System users today.

NextSeq 1000 and NextSeq 2000
P2 Reagents

40 Gb
400 M single reads

Output per flow cell

2 × 50 bp

Quality Score

≥ 85% of bases higher than Q30

Run time

~13 hours

80 Gb
400 M single reads

Output per flow cell

2 × 100 bp

Quality Score

≥ 80% of bases higher than Q30

Run time

~21 hours

120 Gb
400 M single reads

Output per flow cell

2 × 150 bp

Quality Score

≥ 75% of bases higher than Q30

Run time

~29 hours

Applications available

Small whole-genome sequencing
(300 cycles)

130 Mb genome;
> 30× coverage

No. samples

30

Time

~29 hours

Whole-exome sequencing
(200 cycles)

50× mean targeted coverage;
90% targeted coverage at 20×

No. samples

16

Time

~21 hours

Single-cell RNA-Seq
(100 cycles)

4K cells,
12.5K reads/cell

No. samples

8

Time

~13 hours

NextSeq 2000
P3 Reagents

55 Gb
1.1 B single reads

Output per flow cell

2 × 25 bp

Quality Score

≥ 85% of bases higher than Q30

Run time

~11 hours

110 Gb
1.1 B single reads

Output per flow cell

2 × 50 bp

Quality Score

≥ 85% of bases higher than Q30

Run time

~19 hours

220 Gb
1.1 B single reads

Output per flow cell

2 × 100 bp

Quality Score

≥ 80% of bases higher than Q30

Run time

~33 hours

330 Gb
1.1 B single reads

Output per flow cell

2 × 150 bp

Quality Score

≥ 75% of bases higher than Q30

Run time

~48 hours

Applications available

Small whole-genome sequencing
(300 cycles)

130 Mb genome;
> 30× coverage

No. samples

75

Time

~48 hours

Whole-exome sequencing
(200 cycles)

50× mean targeted coverage;
90% targeted coverage at 20×

No. samples

40

Time

~33 hours

Single-cell RNA-Seq
(100 cycles)

4K cells,
12.5k reads/cell

No. samples

20

Time

~19 hours

Specifications based on Illumina PhiX control library at supported cluster densities.