Animal and plant genotyping has become a mainstay of modern agricultural research. Illumina microarrays enable high-throughput screening of known genomic markers to inform selection and breeding decisions. Array-based studies can also be used to discover novel associations between SNP (single nucleotide polymorphism) markers and traits or diseases.
CRISPR-Cas9 is a recently developed genome editing technique that allows scientists to perform precise genomic manipulation quickly and conveniently. This technology has a vast spectrum of applications. As with any molecular biology technique, it is crucial that the obtained results have high levels of specificity. This review highlights recent publications that demonstrate the use of genomic technologies and high-throughput sequencing in CRISPR-Cas9 experiments for checking specificity and off-target effects across the genome.Access PDF
Not every nonhuman organism has been extensively genetically investigated, resulting in an absence of commercial genotyping arrays for certain plant and animal species. We offer custom microarrays that can be designed with all the discovered markers for species of interest, enabling genotyping studies to identify variants associated with desired phenotypic traits. Through ongoing work with major agrigenomics consortia, our technology is being used to identify new, relevant markers in a wide range of species.
A common form of natural diversity, copy number variation (CNV) is also heritable. It appears to play an important role in agrigenomics. CNV differences can correlate to health and production traits, and contribute to phenotypic diversity. In animal and plant genotyping studies, the distribution of high-density probes can be used to identify CNV breakpoints and ultimately accelerate genomic improvements. Studies are ongoing to determine if the impact of CNVs is the result of differences in copy number for key genomic segments, structural modification of the genome, or a combination of both.
Microarray-based animal and plant genotyping can be used to create fine map traits and/or test a genotype-phenotype hypothesis. Quantitative trait loci (QTL) analyses can be performed on the results to characterize how differential gene expression might contribute to phenotypic variation.
Association mapping across an entire animal or plant genome provides insight into the genetic location and architecture of linking traits to species’ adaption (eg, to a wide variety of environmental conditions). The results of these studies support whole-genome selection applications (eg, fingerprinting, net merit, marker-assisted breeding) that enhance the value of commercial crops and herds. Illumina microarrays offer focused content, custom capabilities, and convenient add-on content capabilities.
GeneSeek uses custom Illumina microarrays to genotype cattle, sheep, pigs, and various crops. The resulting genomic information supports selective breeding practices, helping ranchers increase yield and improve herds.Read Interview
A high-precision scanner that supports an extensive range of array applications, including genotyping, methylation profiling, and cytogenetic analysis.View Product
Interrogate virtually any target across any species to create a fully customized genotyping array tailored to your unique study needs.View Product
Infinium XT is a comprehensive microarray solution that enables production-scale genotyping of up to 50,000 single or multi-species custom variants.View Product