Single-cell RNA sequencing provides a high-resolution view of gene expression within complex biological systems. Illumina Single Cell 3′ RNA Prep, powered by PIPseq (particle-templated instant partition sequencing) chemistry, enables a scalable and accessible approach to single-cell analysis.​ This workflow demonstrates how researchers can perform single-cell RNA sequencing using a flexible, microfluidics-free method that simplifies implementation while maintaining high data quality.​ Key features of the workflow include:​ - Microfluidics-free sample preparation using standard laboratory equipment​ - Scalable from small pilot studies to high-throughput experiments ​ - 3′ transcriptome profiling for sensitive gene expression analysis ​ - Consistent, reproducible results enabled by PIPseq chemistry ​ - Seamless integration with Illumina sequencing platforms and downstream analysis with Illumina Connected Multiomics​ By reducing workflow complexity and enabling flexible study design, this approach supports both new and experienced researchers in expanding single-cell RNA sequencing applications.​ For Research Use Only. Not for use in diagnostic procedures Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: https://www.linkedin.com/company/illumina X: https://x.com/illumina Instagram: https://www.instagram.com/illuminainc/ Facebook: https://www.facebook.com/illuminainc TikTok: https://www.tiktok.com/@illuminainc