Instruments

NovaSeq 6000Dx Instrument specifications

IVD-compliant high-throughput sequencing for the clinical lab

Take a closer look at the output, run time, read length, sample throughput, and other NovaSeq 6000Dx specifications

NovaSeq 6000Dx instrument render

Output per flow cell

NovaSeq 6000Dx Instrument Performance Parameters(•,†)

Flow Cell Type Read Length Output Reads Per Run(‡) Run Time Data Quality (¶)
S2 2 x 150 bp ≥ 1 Tb ≥ 6.67 B ≤ 40 hours ≥ 85% Q30
S4 2 x 150 bp ≥ 3 Tb ≥ 20 B ≤ 45 hours ≥ 85% Q30

• Libraries generated with Illumina DNA Prep with Enrichment Dx.
† For performance parameters in RUO mode, see NovaSeq 6000 System specifications.
‡ Paired-end reads per flow cell.
¶ A quality score of Q30 corresponds to an error rate of 1 in 1000 bases called.

Variant calling performance*

NovaSeq 6000Dx Instrument

  Germline variant calling Somatic variant calling
SNV Positive Percent Agreement (%) ≥ 99.8% ≥ 99.8%
Insertion PPA (%) ≥ 97.9% 100%
Deletion PPA (%) ≥ 96.9% 100%
Negative Percent Agreement (%) > 99.9% > 99.9%
Overall Percent Agreement (%) > 99.9% > 99.9%

*Variant calling performance results obtained using a representative assay designed to query a variety of genes covering approximately 2 million bases across 23 different chromosomes, and containing wide GC content (20% to 86%), insertions (up to 18 base pairs), and deletions (up to 21 base pairs).

Instrument specifications

NovaSeq 6000Dx instrument specifications

NovaSeq 6000Dx instrument specifications

  • Instrument configuration
    Computer and touch screen display Installation setup and accessories data collection and analysis software
  • Instrument control computer
    Note: Computer configurations will be upgraded regularly; contact your local account manager for current configuration
    Base Unit: Axiomtek MANO525 with i7-8700T
    CPU Memory: 2 x 8 GB DDR4 SODIMM
    Hard Drive: None Solid-State Drive: 256 GB 2242 M.2
    Operating System: Windows 10
  • Operating environment
    For Indoor Use Only
    Temperature: 19°C-25°C (22°C ±3°C), <2°C change per hour
    Humidity: 20%-80% relative humidity, non-condensing
    Altitude: Below 2000 meters (6500 feet)
    Ventilation: Maximum of 8530 BTU/h and average 6000 BTU/h
  • Laser
    Class 1 laser product embedded with class IV lasers
    532 nm, 660 nm, 780 nm, 790 nm
  • Dimensions with monitor
    W × D × H
    80.0 cm × 94.5 cm × 165.6 cm
    (31.5 in × 37.2 in × 65.2 in)
  • Weight
    481 kg (1059 lb)

    Includes 3.5 kg (7.8 lb) for leak tray and 0.9 kg (2 lb) for keyboard and mouse
  • Crated Weight
    628 kg (1385 lb)
  • Power requirements
    200–240 VAC 50/60Hz, 16A, single phase, 2500 W Illumina provides a region-specific uninterruptible power supply
  • Radio frequency identifier (RFID)
    Frequency: 13.56 MHz
    Power: supply 3.3 volts DC ± 5%, current 120 mA, RF output power 200 mW
  • Network connection
    Dedicated 1 Gb connection between the instrument and data management system. Connect directly or through network
  • Bandwidth for network connection
    200 Mb/s/instrument for internal network uploads
    200 Mb/s/instrument for BaseSpace Sequence Hub uploads
    5 Mb/s/instrument for Instrument Operational Data uploads
View in-depth specs sheet (PDF)

Specifications shown here are for IVD use. View Research Mode only specs, applicable only when running the NovaSeq 6000Dx instrument in Research Mode.

Key technologies

Accurate and efficient data analysis technology

Each NovaSeq 6000Dx instrument comes with a paired dedicated DRAGEN Server and included license for accelerated secondary analysis. With significant advancements in hardware acceleration, lossless compression, and technology innovation, DRAGEN is setting new standards for accuracy and increased genome coverage to deliver actionable insights faster.

Run management technology

Illumina Run Manager for NovaSeq 6000Dx is fully integrated with the sequencing system. Accessed either on-instrument or remotely, this intuitive interface enables control of user and instrument management, application configuration, run setup, and secondary analysis modules on the DRAGEN Server. Plan and start your sequencing runs in IVD and RUO Modes, track libraries with audit trails, and monitor run progress.

Sequencing by synthesis technology

NovaSeq 6000Dx leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.

Patterned flow cell technology

NovaSeq 6000Dx incorporates patterned flow cell technology to generate a high level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.

Intended Use for the NovaSeq 6000Dx Instrument

United States:

The NovaSeq 6000Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NovaSeq 6000Dx instrument is not intended for whole-genome or de novo sequencing. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.

European Union/other:

The NovaSeq 6000Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.

Interested in learning more about the power of NovaSeq 6000Dx?

Talk to an Illumina representative today.