Methylation Sequencing
Benefits of Methylation Sequencing
Cytosine methylation can significantly modify temporal and spatial gene expression and chromatin remodeling. Leveraging the power of next-generation sequencing (NGS), both genome-wide analysis and targeted methylation sequencing approaches can provide researchers with insight into methylation patterns at a single nucleotide level.
Advantages of methylation sequencing:
- Discover methylation patterns of CpG, CHH, and CHG regions across the human genome
- View methylation at practically every cytosine in the genome across most species with whole-genome bisulfite sequencing (WGBS), a genome-wide approach
- Capture full sample diversity with small amounts of DNA
- Cover emerging regions of interest in the human genome, such as those identified by ENCODE, FANTOM5, and the Epigenomics RoadMap Consortium, with targeted methylation sequencing
Advancing DNA Methylation Studies with NGS
This eBook discusses gene expression and regulation research. See how scientists use methylation sequencing and array methods to accelerate their research.
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How Does Bisulfite Sequencing Work?
Many approaches leverage the high quality and sensitivity of NGS for methylation analysis. Most methods rely on bisulfite conversion of DNA to detect unmethylated cytosines. Bisulfite conversion changes unmethylated cytosines to uracil during library preparation. Converted bases are identified (after PCR) as thymine in the sequencing data, and read counts are used to determine the % methylated cytosines.
Bisulfite conversion sequencing can be done with targeted methods such as amplicon methyl-seq or target enrichment, or with whole-genome bisulfite sequencing. Additionally, alternative chemistries like OxBS and TAB-Seq can be used with NGS for identification of hydroxymethylation (5-hMc) in conjunction with methylation (5-mc) analysis.
See the Field Guide to DNA Methylation Analysis for a deep dive into methylation analysis methods.
Methylation Research Articles
Epigenetic Contributions to Addiction
Marilee Morgan discusses the use of targeted methylation sequencing to correlate epigenetic signatures with brain image scans of substance abusers.
Methylation and Metastasis
Researchers use NGS-based methylation sequencing and microarrays to investigate the epigenetics behind cancer metastasis.
Methylation and Exercise
Researchers use RNA-Seq and methylation arrays to study exercise-related epigenetic changes.
Featured Methylation Sequencing Workflow
Access a comprehensive workflow for methylation sequencing, from library preparation to data analysis. Whole-genome bisulfite sequencing provides base-level methylation quantification for all cytosines.
Library Preparation
Sequencing
Data Analysis
Methylation Sequencing Data Analysis Tool
DRAGEN secondary analysis supports an extensive range of applications, from methylation sequencing data analysis to whole-genome, exome, and single-cell RNA-Seq data analysis.
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Related Solutions
Epigenetics and Cancer
Studies of aberrant methylation, altered transcription factor binding, and other epigenetic alterations can provide insight into important tumorigenic pathways. Learn more about cancer epigenetics research.
Complex Disease Genomics
Genome-wide methylation sequencing can help researchers understand the functional mechanisms at work in complex neurological, immunological, and other diseases. Learn more about complex disease research.
Methods Guide
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.
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Featured Publications
The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells.
J Biotechnol 199 38-46 2015
Single-cell DNA methylome sequencing and bioinformatic inference of epigenomic cell-state dynamics.
Cell Rep 10 1386-97 2015
Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors.
Nat Biotechnol 33 769-74 2015
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Additional Resources
Peter Laird: Epigenetics, DNA Methylation, and Arrays
Peter Laird, PhD, discusses his studies of epigenetics and the role DNA methylation may play in contributing to cancer.
Whole-Genome Bisulfite Sequencing Library Prep
Efficient nanogram-scale library preparation, sequencing, and analysis of DNA methylation.
Cellular & Molecular Biology
Broaden cell and molecular biology research beyond the conventional methods of protein-interaction and single-gene functional studies.