AmpliSeq for Illumina SARS-CoV-2 Research Panel

This sequencing panel offers a quick, accurate, and cost-effective method to research the SARS-CoV-2 virus. Read More...
Select Product(s)
Panel

AmpliSeq™ SARS-CoV-2 Research Panel for Illumina®

20020496

Price
 
 

Product Highlights

This research panel contains 247 amplicons in 2 pools targeting the SARS-CoV-2 genome. The panel includes >99% coverage of the Coronavirus genome (~30kb) and covers all potential serotypes.

Community Panel Features

AmpliSeq for Illumina Community Panels are made-to-order. We provide the designs for your convenience; however, they do not have associated performance metrics. For predesigned and tested content, view AmpliSeq for Illumina Ready-to-Use Panels.

AmpliSeq for Illumina Community Panels contain content selected and designed with input from leading disease researchers. You can order this community panel as is, or customize the panel to meet your needs, using Illumina Concierge to add or remove genes and amplicons.

Other Required Products

In addition to the community panel, you will also need an AmpliSeq Library PLUS kit and index adapters.

Additional Information

Frequently Asked Questions

Panel Type and Usage

The AmpliSeq for Illumina SARS-CoV-2 Community Panel, in combination with AmpliSeq for Illumina library prep, index, and accessories, is a targeted RNA/cDNA amplicon assay for epidemiological research of the SARS-CoV-2 virus (Research Use Only). The panel is a 2-pool design, containing 247 amplicons/primer pairs (242 unique amplicons (Pool 1: 125 amplicon, Pool 2: 122 amplicons): 237 viral specific SARS-CoV-2 targets and 5 human gene expression controls) ranging from 125-275 bps in length that covers >99% of the viral genome and all potential serotypes of the virus.

The panel is intended for research use only (RUO) applications, and not for use in diagnostic procedures. No claim or representation is intended to provide information to indicate the panel may be used in diagnosis, prevention, or management of an infection.

Workflow

We recommend customers evaluate commercially available extraction kits capable of isolating viral RNA. The QIAGEN QIAmp Viral RNA Mini Kit (PN 52904) has been demonstrated to be compatible for use in next-generation sequencing library preparation and other testing methods internally. In the event of a back order, any commercially available extraction kit from other companies should be compatible as well.

See the following resources for additional information:

Library preparation for the AmpliSeq for Illumina SARS-CoV-2 Community Panel follows the AmpliSeq for Illumina Custom and Community Panels Reference Guide: Chapter 3 Protocol for RNA Panels (https://support.illumina.com/downloads/ampliseq-for-illumina-custom-and-community-panels-reference-guide-1000000036408.html).

In “Amplify cDNA Targets”, proceed with the following:

  • 2 primer pools for the AMP_RNA program
  • Table 7: 97–192 primer pairs per pool at 18 cycles and 4 minutes
    • Pool 1: 125 amplicons/primer pairs, Pool 2: 122 amplicons/primer pairs
  • Table 8: Adjustments can be made based on sample quality and sample input

Library preparation using the AmpliSeq Library PLUS for Illumina is compatible with all AmpliSeq CD or UD Index Sets for Illumina.

Read depth: 250,000 reads per sample.

Read length: 2 x 151 bp.

Platforms: AmpliSeq for Illumina libraries are compatible with iSeq 100, MiSeq, MiniSeq, and NextSeq systems.

Ordering Information

After adding the panel to your MyIllumina cart and checking out, you will receive your panel order. The panel should state the solution ID: IAACY126664577_100.

1 order = $2223 USD is sufficient for preparing 2250 samples (~$9/amplicon). This does not include AmpliSeq Library PLUS for Illumina, Ampliseq cDNA Synthesis for Illumina, or AmpliSeq CD or UD Indexes for Illumina.

Analysis
Resources and Contact Information

For additional questions please contact techsupport@illumina.com

Frequently Purchased Together

Specifications

Product Comparison

AmpliSeq for Illumina SARS-CoV-2 Research Panel Respiratory Virus Oligo Panel Respiratory Pathogen ID/AMR Enrichment Panel Kit (Powered by Explify) COVIDSeq Test (RUO Version)
Method Amplicon Sequencing Target Enrichment, Target Enrichment, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Amplicon Sequencing, Targeted RNA Sequencing
Nucleic Acid Type RNA RNA, DNA RNA, DNA RNA
Species Category Virus Human, Virus Bacteria, Fungal, Virus Human, Virus
Species Details SARS-CoV-2 (SARS-CoV-2_ MN908947v3) Detects common respiratory viruses, including recent flu strains and SARS-CoV-2. Includes human probes as a quality feature, human probes are included in every sample. Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+). Detects SARS-CoV-2 RNA virus mutations.
Technology Sequencing Sequencing Sequencing Sequencing

Related Products

AmpliSeq for Illumina Comprehensive Panel v3

Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.


AmpliSeq for Illumina Myeloid Panel

Targeted panel to investigate 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers.


AmpliSeq for Illumina Focus Panel

Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.


AmpliSeq for Illumina Immune Response Panel

Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.


AmpliSeq for Illumina Transcriptome Human Gene Expression Panel

Targeted panel that measures expression levels of >20,000 human RefSeq genes.


AmpliSeq for Illumina Library Prep, Indexes, and Accessories

Rapidly prepare amplicon libraries for Illumina sequencers using AmpliSeq for Illumina library preparation and index adapters.