Illumina DNA Prep Product Portfolio

Illumina DNA Prep chemistry uses an innovative bead-transposome complex to tagment genomic DNA by fragmenting and adding adapter tag sequences in a single reaction step. After saturation with input DNA, the bead-based transposome complex fragments a set number of DNA molecules. This bead-based saturation allows the flexibility to use a wide DNA input range and delivers consistent, uniform fragment size distribution, as well as normalized libraries. Following tagmentation, a limited-cycle PCR step adds Illumina DNA Prep-specific index adapter sequences to both ends of a DNA fragment, enabling dual-indexed sequencing of pooled libraries. A subsequent bead-based cleanup step then prepares libraries for use on any Illumina sequencing platform.

Illumina DNA Prep includes all the reagents needed for generating libraries for sequencing, and magnetic sample purification beads for library cleanup steps.

Illumina DNA Prep with Enrichment includes reagents needed for library preparation and enrichment. AMPure XP beads for library cleanup steps, index adapters, and your chosen enrichment oligo panel must be purchased separately.

The following panels are compatible with Illumina DNA Prep for Enrichment:

• TruSight One
• TruSight One Expanded
• TruSight Cancer
• TruSight Hereditary Cancer
• TruSight Cardio

The following index adapters are compatible with Illumina DNA Prep:

• IDT for Illumina Nextera Unique Dual (UD) Indexes (10bp)
• Nextera Combinatorial Dual (CD) Indexes (8bp)

The following index adapters are compatible with Illumina DNA Prep with Enrichment:

• IDT for Illumina Nextera Unique Dual (UD) Indexes (10bp)