Infinium HTS iSelect Methyl Custom BeadChip

Fully customizable methylation microarray for large, targeted epigenetic applications.

5760 samples per week max (with 2 iScan systems, 1 AutoLoader 2x, and 2 Tecan robots over an 8-hour day)

Sample throughput

24 samples per array

Number of samples

500-100,000 user defined CpGs add-on capacity

Number of markers

See full details in the specifications table

Overview

The Infinium Custom Methylation Kit contains the Infinium HTS iSelect Methyl Custom BeadChip plus reagents to enable researchers and commercial users to develop groundbreaking assays for critical epigenetic research applications.

High-throughput workflow

The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.

Customizable content

Customize arrays with up to 100K additional markers to create unique research tools. Select content from existing commercial arrays or epigenetic databases.

Proven Infinium chemistry

The Infinium BeadChip design enables a high degree of methylation probe replication, resulting in high reproducibility, comparable to 100× coverage in methylation sequencing experiments.1

Key features of the Infinium Custom Methylation Kit

  • Customizable content supports up to 100K markers

  • Accurate quantitative measurement of DNA methylation at the single-CpG-level

  • Rapid turnaround time with optional automated bisulfite conversion

Specifications

Required Products

Requires purchase of the Infinium HTS iSelect Methyl Custom BeadChip-24 kit. Each kit contains 2 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 DNA samples.

Requires an iScan Sequencing System.

Optional introductory course for 3 days of hands-on instruction covering all essential steps in the Infinium protocol, including sample and BeadChip preparation, sample scanning, and primary data evaluation and analysis using array analysis software is available for purchase.

/ Results

Applications

The Infinium Custom Methylation Kit allows users to create custom high-throughput assays to measure DNA methylation in targeted regions of the genome for academic epigenetic applications, including common disease research, cancer research, epigenetic aging, and nutriepigenomics.

Example workflow

1
Method
2
Scan
3
Analyze

Related applications and methods

Compare

Infinium HTS iSelect Methyl Custom BeadChip Infinium Methyl EX iSelect Custom BeadChips
Assay type Infinium HTS Infinium EX
Automation capability Automated array loader Automated array loader, Liquid handling robot(s)
Description Using the DesignStudio Assay Design Tool, this product allows labs to create high-throughput, custom, 24- or 48-sample BeadChips to efficiently and accurately assess methylation for up to 100,000 CpG sites in human and select nonhuman samples. This product allows labs to create high-throughput, custom, 24- or 48-sample BeadChips to efficiently and accurately assess methylation for up to 100,000 CpG sites in human and select nonhuman samples.
Input quantity 250 ng DNA 250 ng
Instruments iScan System iScan System
Method Methylation array Methylation array
Nucleic acid type DNA DNA
Number of markers 500-100,000 user defined CpGs add-on capacity 500⁠–100,000 user defined CpGs add-on capacity
Number of samples 24 samples per array 48 sample and 24 sample beadchips available
Sample throughput 5760 samples per week max (with 2 iScan systems, 1 AutoLoader 2x, and 2 Tecan robots over an 8-hour day) 16,128 (per week for 48 sample beadchip)
Specialized sample types Blood, Cell-free DNA, Saliva Blood
Species category Mouse, Human Bovine, Mouse, Canine, Human, Equine, Feline
Species details Select non-human species available on DesignStudio only Select non-human species available on DesignStudio only
Technology Microarray Microarray
Variant class Differentially methylated cytosines

Selection tools

Figures

Infinium I and II assays allow for broader coverage of CpG sites

Illumina methylation BeadChips employ both Infinium I and Infinium II assays, enhancing the overall breadth of coverage. (A) Infinium I probe designs use 2 bead types per CpG locus, 1 each for the methylated and unmethylated states. (B) Infinium II probe designs use 1 bead type for both states.

Design and manufacturing process

Researchers work with Illumina technical design and customer service teams to refine the BeadChip marker content and coordinate the ordering and production process.

  1. Zhou L, Ng HK, Drautz-Moses DI, et al. Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing. Sci Rep. 2019;9(1):10383. Published 2019 Jul 17. doi:10.1038/s41598-019- 46875-5

Resources

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Discover the Flexible, Scalable Infinium Workflow

Custom HTS kits (1)

Infinium Methyl Custom-24 Kit (48 Spl)

20042146

Infinium HTS iSelect Methyl Custom Beadchip, 48 samples

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Custom EX kits (5)

Inf Methyl EX iSelect-48 Kit (48 Spl)

20139002

This kit contains 1 custom BeadChip and reagents to process 48 samples throught the Infinium EX Methylation workflow.

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Inf Methyl EX iSelect-48 Kit (96 Spl).

20139003

This kit contains 2 custom BeadChips and reagents to process 96 samples throught the Infinium EX Methylation workflow.

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Inf Methyl EX iSelect-48 Kit (1152 Spl).

20139004

This kit contains 24 custom BeadChip and reagents to process 1152 samples throught the Infinium EX Methylation workflow.

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Inf Methyl EX iSelect-24 Kit (48 Spl).

20139005

This kit contains 2 custom BeadChip and reagents to process 48 samples throught the Infinium EX Methylation workflow.

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Inf Methyl EX iSelect-24 Kit (96 Spl).

20139006

This kit contains 4 custom BeadChip and reagents to process 96 samples throught the Infinium EX Methylation workflow.

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Services (1)

Infinium® Assay: An Introduction – Customer Site

20015273

Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software.

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Selection summary

Product

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FAQs

 The Infinium HTS iSelect Custom BeadChip includes the standard SNP controls available on the Methylation EPIC BeadChip.

For methylation QC, GenomeStudio 2011, Methylation Module, or DRAGEN Array Methyl QC can be used for human species.

For downstream methylation analysis or analysis of non-human species, SeSAMe (https://www.bioconductor.org/packages/release/bioc/html/sesame.html) or other third party bioconductor tools are recommended at this time.

No, Illumina LIMS does not support Infinium HTS iSelect Methyl Custom BeadChips at this time.

No, FFPE samples are not currently supported.

The Zymo Research EZ-96 Methylation-Lightning MagPrep Kit in manual and automated formats is supported for use upstream to Infinium Methylation assays. Review results in this technical note for more details. 

/ Results

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References

  1. Zhou L, Ng HK, Drautz-Moses DI, et al. Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing. Sci Rep. 2019;9(1):10383. Published 2019 Jul 17. doi:10.1038/s41598-019- 46875-5

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