NovaSeq 6000 Reagent Kits v1.5 offer numerous benefits including an improved Q30 score, support for unique molecular indexes (UMIs), an extended shelf life of six months, and support for Illumina DNA PCR-Free Library Prep.
|NovaSeq 6000 System|
|Flow Cell Type||SP||S1||S2||S4|
|Output Per Flow Cell|
|1 × 35 bp||N/A ‡||N/A ‡||N/A ‡||280-350 Gb|
|2 × 50 bp||65–80 Gb||134–167 Gb||333–417 Gb||N/A ‡|
|2 × 100 bp||134–167 Gb||266–333 Gb||667–833 Gb||1600–2000 Gb|
|2 × 150 bp||200–250 Gb||400–500 Gb||1000–1250 Gb||2400–3000 Gb|
|2 x 250 bp||325-400 Gb||N/A ‡||N/A ‡||N/A ‡|
|Clusters Passing Filter|
|Single Reads||650–800 million||1.3–1.6 billion||3.3–4.1 billion||8–10 billion|
|Paired-end Reads||1.3–1.6 billion||2.6–3.2 billion||6.6–8.2 billion||16–20 billion|
|Run Time §|
|1 × 35 bp||N/A ‡||N/A ‡||N/A ‡||~14 hr|
|2 × 50 bp||~13 hr||~13 hr||~16 hr||N/A ‡|
|2 × 100 bp||~19 hr||~19 hr||~25 hr||~36 hr|
|2 × 150 bp||~25 hr||~25 hr||~36 hr||~44 hr|
|2 x 250 bp||~38 hr||~N/A||~N/A||~N/A|
* Output and read number specifications based on a single flow cell using Illumina PhiX control library at supported cluster densities. The NovaSeq 6000 system can run 1 or 2 flow cells simultaneously.
§ Run time includes cluster generation, sequencing, and base calling. Run times are based on running 2 flow cells of the same type; starting two different flow cells will impact run time.
Specifications based on Illumina PhiX control library at supported cluster densities.
‡ N/A: not applicable
If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X system, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 system. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.