AmpliSeq for Illumina Immune Response Panel

Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.

6 hours (library prep only; doesn't include library quantification, normalization, or pooling time)

Assay time

< 1.5 hr

Hands-on time

1–100 ng (10 ng recommended per pool)

Input quantity

See full details in the specifications table

Overview

The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing research assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor–immune system interactions.

Panel features

  • Relevant Gene Content - Targeted expression of 395 cancer biomarkers indicative of immunotherapy response

  • Fast, Streamlined Workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality RNA or 10 ng of formalin-fixed, paraffin-embedded (FFPE) RNA input samples

  • Accurate Data - Detect expression of genes involved in tumor-immune interactions

The Immune Response Panel is part of an integrated RNA-to-annotated variant solution that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel detects biomarkers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Specifications

Required Products

The AmpliSeq for Illumina Immune Response Panel workflow requires the Immune Response Panel, Library PLUS kit, and index adapter. Also, the cDNA synthesis kit is required to convert total RNA to cDNA.

/ Results

Applications

Example workflow

Project recommendations

Instrument Recommended number of samples Read length
iSeq 100 System

4 samples per run (assumes 1 million reads per sample)

2 × 150 bp

MiniSeq System

Samples per run: mid output: 8, high output: 24 (assumes 1 million reads per sample)

2 × 150 bp

MiSeq System

Samples per run (by reagent kit version): v2 micro: 4, v2: 15, v3: 24 (assumes 1 million reads per sample)

2 × 150 bp

Related applications and methods

Panel (1)

AmpliSeq™ Immune Response Panel for Illumina®

20019169

Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.

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Library prep (3)

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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Index adapters (6)

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.

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Accessory products (3)

Ampliseq™ cDNA Synthesis for Illumina®

20022654

Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.

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AmpliSeq™ Library Equalizer for Illumina®

20019171

Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.

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Selection summary

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