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Pillar oncoReveal Multi-Cancer CNV + RNA Fusion Panel

A pan-cancer panel with combined DNA/RNA content, utilizing the single-tube SLIMamp workflow for fast library prep and turnaround time.

48 hr from sample to report

Assay time

~3 hr

Hands-on time

5–80 ng DNA, 10–50 ng RNA

Input quantity

See full details in the specifications table

Overview

The oncoReveal Multi-Cancer with CNV and RNA Fusion Panel is a combined DNA/RNA multi-cancer panel. The assay combines the DNA-based oncoReveal Multi-Cancer v4 with CNV Panel with the oncoReveal Multi-Cancer RNA Fusion v2 Panel allowing for joint sequencing. The assay uses proprietary Stem Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

  • Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

  • Achieve variant detection as low as 1% VAF without UIDs, even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce “no calls,” repeat testing and difficult interpretation decisions

  • In under 8 hours from DNA to sequencer and under 9–10 hours from RNA to sequencer

Specifications

Assay time 48 hr from sample to report
Cancer type Pan-cancer, Solid tumor
Content specifications 78-gene small pan-tumor oncology panel
Description 78- gene amplicon-based panel with DNA & RNA content, simple single tube workflow
Hands-on time ~3 hr
Input quantity 5–80 ng DNA, 10–50 ng RNA
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 Plus System, MiSeq i100 System
Mechanism of action Multiplex PCR using tiled amplicon
Method Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing
Nucleic acid type DNA, RNA
Number of amplicons Pool 1 (DNA): 341 amplicons. Pool 2 (RNA): >80 amplicons plus MET exon 14 skipping
Number of reactions 24 DNA/24 RNA
On-target reads 99.5% ± 0.1%
Specialized sample types Blood, Low-input samples, FFPE tissue
Species category Human
Target insert size Average: 120 bp (range 86-185 bp)
Technology Sequencing
Uniformity 98.2% ± 0.7%
Variant class Gene fusions, Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Required products

Pillar oncoReveal panels require the use of a Pillar index kit.

 

What accessories do I need?

Pillar oncoReveal panels require the use of a Pillar index kit. Pick your preferred number of reactions.

/ Results

Applications

Example workflow

1
Prep

Pillar oncoReveal Multi-Cancer CNV + RNA Fusion Panel

2
Sequence
3
Analyze

Documentation

Product literature
Support documentation

Figures

Gene list

Pillar oncoReveal Multi-Cancer CNV + RNA Fusion Panel interrogates variants across 60 genes from DNA and 18 driver genes from RNA.

Panel specifications

Pillar oncoReveal Multi-Cancer CNV + RNA Fusion Panel specifications for interrogation of DNA and RNA from tissue biopsy.

oncoReveal™ Multi-Cancer CNV + Fusion

HNA-HS-1001-48

oncoReveal™ Multi-Cancer CNV + Fusion

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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

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Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

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