To provide greater flexibility in ordering single components for library preparation, we are moving to a modular configuration. Our existing TruSeq Nano DNA Library Prep Kits (Cat. No. FC-121-4001, FC-121-4002, and FC-121-4003) will transition to TruSeq DNA Nano (Cat. No. 20015964 for 24 samples and Cat. No. 20015965 for 96 samples) for library preparation. Index adapters will be sold separately as TruSeq DNA Single Indexes (Cat. No. 20015960 for Set A and Cat. No. 20015961 for Set B) and TruSeq DNA CD (combinatorial dual) Indexes (Cat. No. 20015949).

TruSeq DNA Nano

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.Read More...
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Library Prep

TruSeq Nano DNA Low Throughput Library Prep Kit (24 samples)

20015964

Price
 
 

TruSeq Nano DNA High Throughput Library Prep Kit (96 samples)

20015965

Price
 
 

Index Adapters

TruSeq DNA Single Indexes Set A (12 Indexes, 24 Samples)

20015960

Price
 
 

TruSeq DNA Single Indexes Set B (12 Indexes, 24 Samples)

20015961

Price
 
 

TruSeq DNA CD Indexes (96 Indexes, 96 Samples)

20015949

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples)

20020590

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples)

20022370

Price
 
 

Illumina Advantage Products

TG TruSeq® Nano DNA LT Sample Prep Kit, Set A (24 Samples)

TG-202-1001

Price
 
 

TG TruSeq® Nano DNA LT Sample Prep Kit, Set B (24 Samples)

TG-202-1002

Price
 
 

TG TruSeq® Nano DNA HT Sample Preparation Kit (96 Samples)

TG-202-1003

Price
 
 

TG Truseq® Nano DNA Accessory Kit

TG-280-1001

Price
 
 
Accessory Products

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Price
 
 

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Price
 
 

Product Highlights

TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

  • Designed for low sample input
  • High coverage quality
  • Accelerated library prep
Manual preparation of high-quality libraries in less than a day

TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexible throughput options
  • TruSeq DNA Nano with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA Nano with 96 CD Indexes  supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

View a list of automation vendors with robotic systems that support the 96-sample workflow

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (based on 30× coverage of a human genome) 350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp
HiSeq 2500 System 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome)  Rapid run, 350 bp insert: ≤ 2 × 100 bp
Rapid run, 550 bp insert: ≤ 2 × 250 bp
High output, 350 bp insert: ≤ 2 × 100 bp
High output, 550 bp insert: ≤ 2 × 125 bp

Product Comparison

TruSeq DNA Nano TruSeq DNA PCR-Free Nextera DNA Flex Library Prep Kit
Assay Time ~6 hours total assay time 5 hours total assay time ~3-4 hours (from DNA extraction to normalized library)
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Description A low-input research method that delivers high genome coverage quality and reduced bias. A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more.
Hands-On Time ~4 hours 4 hours 1-1.5 hours
Input Quantity 100 ng genomic DNA 1 ug DNA Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA.
Mechanism of Action Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Bead-linked transposome
Method Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing
Multiplexing Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 96 available indexes
Specialized Sample Types Low-Input Samples, Not FFPE-Compatible Not FFPE-Compatible Blood, Not FFPE-Compatible, Saliva
Species Category Human, Mammalian, Mouse, Other, Plant, Rat Human, Mammalian, Mouse, Other, Plant, Rat Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish
Species Details Compatible with most large DNA genomes. Compatible with most large DNA genomes. Compatible with any species
Target Insert Size 350 bp or 550 bp 350 bp or 550 bp ~500 bp

Method-Specific Workflow Example

 

Customer Stories

Unmasking the Viral Etiology of Cancer and Immune Disease

Karolinska Institutet researchers use Illumina sequencers to identify novel HPV types associated with non-melanoma skin cancers.

Read More
BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace Sequence Hub SRA Submission App enables tracking of samples, from library prep to data analysis, helping the Biotechnology Resource Center Sequencing Core (BRC-Seq) at the University of British Columbia deliver high-quality sequencing data and additional value to its customers.

Read More
Comparison of TruSeq DNA Library Preparation Workflows
Specification TruSeq DNA Nano TruSeq DNA PCR-Free
Kit type TruSeq DNA Nano LT & HT TruSeq DNA PCR-Free LT & HT
Description Based upon widely adopted TruSeq library prep, with lower input and improved data quality Superior genomic coverage with radically reduced library bias and gaps
Workflow LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available
LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available
Input quantity 100–200 ng
1–2 μg
Includes PCR Yes No
Assay time 5.5 hours ~5 hours
Hands-on time 4 hours ~4 hours
Target insert size 350 bp or 550 bp 350 bp or 550 bp
Gel-free Yes Yes
Number of samples supported 24 (LT) or 96 (HT) samples 24 (LT) or 96 (HT) samples
Size-selection beads Included Included
Applications Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies
Sample multiplexing Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations
Compatible Illumina sequencers MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems
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