TruSeq DNA Nano

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.

~6 hr total assay time

Assay time

~4 hr

Hands-on time

100 ng genomic DNA

Input quantity

See full details in the specifications table

Illumina DNA Prep and Illumina DNA PCR-Free Prep are available. These library prep solutions offer improved performance while reducing turnaround time and hands-on time compared to TruSeq DNA Nano.

Overview

TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Key features

  • Designed for low sample input

  • High coverage quality

  • Accelerated library prep

Manual preparation of high-quality libraries in less than a day

TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexible throughput options

  • TruSeq DNA Nano with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA Nano with 96 CD Indexes  supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

Fast, Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.


Specifications


Required products

Illumina now offers modular product ordering to enable flexibility in your workflows. You may order any of the following library prep products with any of the following indexes. 

Illumina Advantage large-scale sequencing products offer the highest level of service and support to ensure operational success. They feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater lab efficiency. 

The Illumina Free Adapter Blocking Reagent is an optional enzymatic solution designed to block free adapters from library pools, to minimize the risk of index hopping.

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Applications

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
NextSeq 550 System

1 sample per run (based on 30× coverage of a human genome)

350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp


Related applications and methods

How others use this product

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TruSeq DNA Nano TruSeq DNA PCR-Free Illumina DNA Prep
Assay time

~6 hr total assay time

5 hr total assay time ~3-4 hr (from DNA extraction to normalized library)
Automation capability Liquid Handling Robots Liquid Handling Robots
Automation details Explore available automation methods Explore available automation methods Explore available automation methods
Description A low-input research method that delivers high genome coverage quality and reduced bias. A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome.

A fast, flexible library prep workflow that accommodates an assortment of sample types and DNA input amounts, allowing access to a wide range of applications, from human to microbial whole-genome sequencing and more. 

Hands-on time ~4 hr 4 hr 1-1.5 hr
Input quantity 100 ng genomic DNA 1 ug DNA 1-500 ng DNA for small genomes (e.g. microbial), or 100-500 ng DNA for large genomes (e.g. human). (For blood and saliva, see the reference guide).
Instruments MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode
Mechanism of action Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Bead-linked transposome
Method Shotgun Sequencing, Whole-Genome Sequencing, Genotyping by Sequencing Shotgun Sequencing, Whole-Genome Sequencing, Genotyping by Sequencing Shotgun Sequencing, Whole-Genome Sequencing, De Novo Sequencing, Amplicon Sequencing
Multiplexing Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
Nucleic acid type DNA DNA
Specialized sample types Not FFPE-Compatible, Low-Input Samples Blood, Not FFPE-Compatible, Saliva
Species category Other, Mammalian, Mouse, Human, Rat, Plant Other, Mammalian, Mouse, Human, Rat, Plant Drosophila, Any species, Mammalian, Mouse, Yeast, Zebrafish, Human, Rat, Plant, Virus, Nematode, Bacteria
Species details Compatible with most large DNA genomes. Compatible with most large DNA genomes. Compatible with any species
Target insert size 350 bp or 550 bp 350 bp or 550 bp ~350 bp
Technology Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs)

Selection tools:

Library prep (1)

TruSeq Nano DNA Low Throughput Library Prep Kit (24 samples)

20015964

Includes reagents and Sample Purification Beads for preparing 24 libraries. Purchase index adapters separately.

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TruSeq Nano DNA High Throughput Library Prep Kit (96 samples)

20015965

Includes reagents and Sample Purification Beads for preparing 24 libraries. Purchase index adapters separately.

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Index adapters (1)

IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)

20040870

Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Nano and TruSeq PCR-Free Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022370, with no changes to the remaining 184. Purchase library preps and probe panels separately.

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Illumina Advantage Products (3)

TG TruSeq® Nano DNA LT Sample Prep Kit, Set A (24 Samples)

TG-202-1001

The Kitted reagents for DNA Sample Preparation of 24 total samples for Single-Read, Paired-End, and Multiplex sequencing. This kit contains 12 unique single indexes.(Indexes 2,4,5,6,7,12,13,14,15,16,18,19)

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TG TruSeq® Nano DNA LT Sample Prep Kit, Set B (24 Samples)

TG-202-1002

The Kitted reagents for DNA Sample Preparation of 24 total samples for Single-Read, Paired-End, and Multiplex sequencing. This kit contains 12 unique single indexes. (Indexes 1,3,8,9,10,11,20,21,22,23,25,27)

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TG Truseq® Nano DNA Accessory Kit

TG-280-1001

Accessory kit for use with the TruSeq Nano DNA LT Kits A and B and cfDNA workflow. One Accessory kit provides enough addiitonal EPM reagent for 4 additional 16 sample batches through the cfDNA workflow.

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Selection summary

Product

Qty

Unit Price

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

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