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HiSeq 2500 System

Even More Data, Faster
Even More Data, Faster

HiSeq v4 reagents increase yield and throughput.
BioPharma QC
BioPharma QC

See how Merck Serono uses Illumina sequencing for biological quality control (QC) applications in biopharma production.
High-Throughput Library Prep Automation
High-Throughput Library Prep Automation

Save time and expense by taking advantage of Illumina qualified library prep automation methods.

Flexibility for Multiple Applications

The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports a broad range of applications and study sizes. Choose between rapid run and high output modes to fit your project needs.

Next: Rapid Run Mode Produces Quick Results

Rapid Run Mode Produces Quick Results

Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 250 base pair reads. The longer reads enable greater depth of coverage, improved assembly for de novo applications and aid in long-read applications such as metagenomic analyses.

Next: High Output Mode for Larger Studies

High Output Mode for Larger Studies

High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process over 6× more samples than rapid mode and to complete large projects quickly and efficiently. HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.

Start Over: Flexibility for Multiple Applications
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Workflow

 
Prep

Access optimized workflows for preparing DNA or RNA sequencing libraries.

 
Sequence

Easily switch between rapid turnaround time and high output modes to suit your research needs.

 
Analyze

Quickly go from raw data to meaningful results with a suite of analytical tools.

 
Share

Store and share data with colleagues.

See HiSeq 2500 Application-Specific Workflows

System Specifications

9 Gb–1 Tb
output range

300 M–4 B
reads per run

2 × 250 bp
max read length

View All HiSeq 2500 Specifications

Customer Stories

Progress in Rapid Identification of Variants Linked to Genetic Diseases
Progress in Rapid Identification of Variants Linked to Genetic Diseases

Learn how DNA sequencing is leading to progress in the rapid identification of variants that might be involved in genetic diseases. Thanks to this work, Dr. Kingsmore and his team now hold a Guinness World Records title.

View Video
Exploring the Genetic Basis of Oral Cancer

Researchers in India study a deadly oral cancer that is strongly associated with chewing tobacco together with slaked lime.

Read Article
NGS Identifies Rare Disease Variants in Infants with Undiagnosed Disease

Landmark NGS study leads to a national clinical diagnostic exome sequencing platform in Singapore.

Read Article
Read More Stories
Decade of Sequencing Graphic

One Decade of Sequencing

Explore the breakthroughs, advancements, and progress

Learn More
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Featured Products

HiSeq Rapid SBS Kit v2
HiSeq Rapid SBS Kit v2

HiSeq Rapid v2 SBS Kits enable the longest reads on HiSeq instruments, with reads up to 2x250 bp. The new kits generate up to 300 Gb of data per run. HiSeq Rapid SBS Kits v2 are the ideal choice for sequencing on HiSeq systems.

Learn More
TruSeq DNA Exome
TruSeq DNA Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.

Illumina DNA Prep with Enrichment
Illumina DNA Prep with Enrichment

A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.

HiSeq SBS Kit v4
HiSeq SBS Kit v4

Reagents for determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on enabled HiSeq systems.

View All HiSeq 2500 Products

Related Solutions

For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Innovative technologies

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

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