NovaSeq 6000 Sequencing System

Join other NovaSeq owners in the NovaSeq Online Community. Collaborate with Illumina moderators and NovaSeq owners. Discuss best practices, troubleshoot, and learn about how others are using NovaSeq preparation kits, push-button sequencing, and automated data analysis to fuel their research.
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Illumina NovaSeq Community

4800-6000 Gb

output range

32-40 B

reads per run

2 × 250 bp

max read length

View All NovaSeq Specifications

*Specifications based on a dual flow cell run of S4 flow cells.

Regeneron: Scaling NGS to Deliver More Discoveries, Faster

Aris Baras and John Overton of Regeneron discuss their latest work and overall mission to explore new variations in genomes that can help explain disease. Hear how they plan to utilize the NovaSeq 6000 System.

Chan Zuckerbeg Biohub: Conducting Innovative Experiments with NGS

Steve Quake and Joe DeRisi discuss their mission to conduct innovative experiments and new kinds of collaborations in genomics. Their recent partnering with Stanford, UCSF and Berkeley enables research that may improve human health.

Exploring the Unknown: NGS Supports Species Research

The NovaSeq 6000 System is enabling OIST researchers to sequence and study various ocean and land species.

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Exploring the Unknown: NGS Supports Species Research
Decade of Sequencing Graphic

One Decade of Sequencing

Explore the breakthroughs, advancements, and progress

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NovaSeq Reagent Kits
NovaSeq Reagent Kits

Reagent kits for the NovaSeq 6000 System provide ready-to-use cartridge-based reagents for cluster generation and SBS.

TruSeq DNA PCR-Free
TruSeq DNA PCR-Free

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.

TruSeq Stranded Total RNA
TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.