NovaSeq 6000 Sequencing System
Drive your discoveries here
The NovaSeq 6000 Sequencing System is designed to adapt to your needs. This robust, scalable system has been adopted by leading research centers and featured in countless publications. Impactful discoveries are within reach.
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Scalable throughput for dynamic study sizes
So many ways to sequence. Scale up and down with a tunable output of up to 6 Tb and 20B single reads in < 2 days.
Broad, deep sequencing for clear answers
Accelerate your science with reliable sequencing breadth and depth for every project.
Flexible performance for mix and match options
Do what you want. Mix and match flow cell types, run one or two flow cells at a time, choose between multiple read lengths, workflows, and more.
Australia’s Koala Conservation program uses Illumina whole genome sequencing to save species
Dr. Carolyn Hogg and Professor Kathy Belov are researchers from the University of Sydney, Australia who are using whole genome sequencing of rare and endangered species with aims to provide conservation plans for the species ongoing survival.
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Advanced applications. Impactful discoveries.
The NovaSeq 6000 system offers deep and broad coverage through advanced applications for a comprehensive view of the genome. Unlock a full spectrum of genetic variation and biological function with high-throughput sequencing.
See All ApplicationsThe NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. Its tunable output generates up to 6 Tb and 20B single reads in dual flow cell mode with streamlined workflows. Configure the system to sequence up to 48 genomes in ~2 days with comprehensive coverage.
Prep
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing.
llumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Illumina Complete Long Read Prep, Human
Highly accurate, high-performance full workflow solution for comprehensive human WGS with long-read data from NovaSeq platforms.
Sequence
NovaSeq 6000 Reagent Kits v1.5
Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.
Analyze
*Time per sample, assuming 30x coverage/sample
DRAGEN Germline Pipeline (on BaseSpace Sequence Hub or on DRAGEN server)
The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both.
DRAGEN Somatic Pipeline (on BaseSpace Sequence Hub or on DRAGEN server)
The DRAGEN Somatic Pipeline aligns and variant calls Tumor-only or Tumor/Normal FASTQ files, outputting BAM(s) and a VCF.
DRAGEN Illumina Complete Long Read WGS
The DRAGEN Illumina Complete Long Read WGS app generates our most accurate and comprehensive whole human genome data using DRAGEN and Illumina Complete Long Reads.
Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq 6000 System offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell.
Prep
*2.5 days total prep time, 6 hours hands-on time
Illumina DNA Prep with Enrichment
This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 100x coverage/sample
DRAGEN Enrichment
This app aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, or both.
Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. With the NovaSeq 6000 System, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell.
Prep
Illumina Stranded Total RNA Prep
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 200x coverage/sample
DRAGEN RNA Pipeline
Performs alignment, quantification, and fusion detection.
Tried-and-true performance
System specifications
Output range
80 - 6000 Gb
Paired end reads per run
1.6B - 40B
Max read length
2 × 250 bp
Run time
13 - 44 hours
A flexible workflow designed to help you accelerate your research and maximize results
Manage Workflow
Manage your workflow seamlessly with BaseSpace Clarity LIMS or your existing LIMS system.
Prepare Libraries
Prepare libraries with a broad range of high-performance library preparation kits.
Sequence
Generate sequences with ready-to-use reagents featuring proven Illumina sequencing by synthesis chemistry.
Analyze Data
Leverage the Illumina DRAGEN Bio-IT Platform or BaseSpace Sequence Hub for accurate, rapid secondary analysis.
Interpret Results
Interpret results, get insights, and drive discovery with BaseSpace Variant Interpreter, and TruSight Software Suite.
Compare with NovaSeq X Series & NovaSeq 6000
| NovaSeq 6000 System | NovaSeq X Series | |
|---|---|---|
| Key Features: |
|
|
| Run Time: | ~13 hr - ~44 hr | ~13 hr - ~48 hr |
| Maximum Output: | 6 Tb | 16 Tb (for NovaSeq X Plus system) |
| Maximum Reads per Run: | 20 billion single-end reads; 40 billion paired-end reads | 52 billion single-end reads; 104 billion paired-end reads (for NovaSeq X Plus system) |
| Maximum Read Length: | 2 x 250 bp | 2 x 150 bp |
| Key Applications: | Whole-genome sequencing Whole-exome sequencing Whole-transcriptome sequencing |
Whole-genome sequencing Whole-exome sequencing Whole-transcriptome sequencing |
| DRAGEN Data Analysis Offerings: | (on-premise or cloud) DRAGEN Germline DRAGEN Enrichment DRAGEN Somatic DRAGEN RNA |
(onboard) DRAGEN Germline DRAGEN Enrichment DRAGEN RNA DRAGEN Illumina Complete Long Read WGS |
| Featured Products: | NovaSeq 6000 Reagent Kits NovaSeq XP Workflow Illumina RNA Prep with Enrichment Illumina DNA PCR-Free Prep Illumina Complete Long Read Prep, Human |
Novaseq X Series Reagent Kits Illumina DNA PCR-Free Prep Illumina DNA Prep with Enrichment Illumina Stranded Total RNA Prep |
Expand Your Access to More Data and Applications
High-Throughput Sequencing
Probe deeper into a single sample for insights or process more samples to improve statistical signficance.
Multiomics
Perform transformative science with access to multiple emerging high-throughput omics applications.
Long-Read Sequencing Technology
Long-read sequencing enables the sequencing of much longer DNA fragments than traditional short-read sequencing methods.
Support for your science
Products & Services
NovaSeq 6000 v1.5 Reagent Kits
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
NovaSeq 6000 Xp Workflow
The NovaSeq 6000 Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 system flow cell, without additional instrumentation.
Illumina RNA Prep with Enrichment
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
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Customer Stories
Go behind the science
See what’s possible with the proven NovaSeq 6000 system and NGS technology
See All StoriesRady Children’s Institute for Genomic Medicine is using the DNA PCR-Free Prep kit for NovaSeq 6000 to make a potentially record-breaking time-to-diagnosis for children with rare genetic disorders
Read MoreOmniSeq, Inc. scaled up to process a large number of both tissue and blood plasma samples every week, batching from 16 to 192 samples in a single run on the NovaSeq 6000
Read MoreGenomics England’s human genome initiative (GenOMICC) has launched a study aimed at uncovering genetic factors in susceptibility to COVID-19
Read MoreThe DRAGEN platform enables GeneDx to scale to whole-genome analysis and identify variants with precision
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