Comprehensive coverage for genome-wide DNA methylation studies

Quantitatively interrogate methylation sites across the genome at single-nucleotide resolution with methylation microarrays

Methylation Array Analysis

DNA methylation plays an important and dynamic role in regulating gene expression. It allows cells to suppress expression of viral and nonhost DNA elements, and facilitates response to environmental stimuli. Aberrant DNA methylation (hyper- or hypomethylation) and its impact on gene expression have been implicated in many biological processes and diseases. Methylation arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.

Illumina Methylation Arrays

Infinium Methylation allows you to interrogate CpG sites at single-nucleotide resolution, providing highly robust measurements of DNA methylation. Choose from one of our curated genome-wide arrays, or design your own beadchip for targeted applications.

Human MethylationEPIC BeadChip

Powered by the Infinium assay, the MethylationEPIC BeadChip is ideal for epigenome-wide association studies (EWAS), tumor profiling, and various cell biology experiments. It offers comprehensive, expert-selected coverage, including 99% of RefSeq genes, 95% of CpG islands, high coverage of enhancer regions, and other content categories. With > 90% of the original content from the Infinium HumanMethylation450K covered, the MethylationEPIC kit supports the next generation of epigenetics research.

Mouse Methylation BeadChip

Also leveraging the Infinium assay, the Mouse Methylation BeadChip can be used in EWAS and other experiments probing the methylome in healthy and diseased tissues. Much like the MethylationEPIC BeadChip, the Mouse Methylation BeadChip offers genome-wide coverage, with probes targeted to CpG islands, enhancer regions, transcription start sites, and other important regions of the methylome.

HTS iSelect Methyl Custom BeadChip

Custom Methylation Beadchips provide the ultimate flexibility and cost-savings for targeted research and commercial applications. Custom beadchips can be designed with between 3,000 and 100,000 markers for a variety of applications. The high-throughput 24-sample beadchip reduces per sample and processing costs while maintaining the same robust methylation measurements provided by the Infinium assay.

Our methylation microarrays combine comprehensive coverage and high-throughput capabilities. Advantages include:

  • Comprehensive genome-wide coverage
    • CpG islands
    • Non-CpG and differentially methylated sites
    • Enhancers
    • Open chromatin
    • Transcription factor binding sites
    • miRNA promoter regions
  • Assay reproducibility
    • > 98% reproducibility between technical replicates for all Infinium methylation arrays
    • 98% for the same samples on the legacy HumanMethylation450K array vs. the MethylationEPIC array
    • MethylationEPIC contains > 90% of the HumanMethylation450K content
  • User-friendly, streamlined workflow
  • MethylationEPIC has been validated with FFPE samples
iScan

Our methylation arrays follow a user-friendly, streamlined workflow, enabling processing of up to 96 samples simultaneously from low sample input (as little as 250 ng).

Offering quantitative measurement at the single-CpG-site level, these methylation microarrays offer powerful resolution for understanding epigenetic changes.

Click on the below to view products for each workflow step.

MethylationEPIC BeadChip

Robust methylation profiling microarray with extensive coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies and other experiments.

Infinium Mouse Methylation BeadChip

Features > 285k markers across the methylome for high resolution epigenetic analyses of diverse murine strains.

Infinium Custom Methylation Kit

Use this kit to create made-to-order, high-throughput assays to fit your specific needs. The flexibility and features of the Infinium HTS iSelect Methyl Custom BeadChip make it a powerful tool for a wide range of epigenetic applications.

iScan System

Supports high-throughput BeadChip processing, scanning hundreds to thousands of samples quickly and accurately.

GenomeStudio Software and Methylation Module

Visualize Infinium controls to ensure quality processing and perform basic analysis on DNA methylation beadchip data.

Explore a variety of methods used to detect cytosine modifications, including methylation arrays, and learn more about the impact of cytosine methylation.

Read Guide
Field Guide to Methylation Methods
Methylome and Clinical Outcomes: An Interview with Dr. Kathleen C. Barnes

In this wide-ranging interview, Dr. Kathleen C. Barnes describes her work as a genetic epidemiologist, how COVID-19 impacted her research plans, and how methylation arrays add another dimension to the study of infectious diseases.

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Scouring The Epigenome For The Origins Of Dementia and Mental Illness

Dr. Mill at King’s College London uses methylation arrays to identify epigenetic changes that contribute to neurodegenerative disorders.

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Epigenetics Studies Uncover Obesity-Driven Methylation Signatures

Dr. Samani at University of Leicester uses methylation arrays to identify cell signaling disruption linked with high BMI.

Read Interview
Complex Disease Studies
Methylation Profiling for Complex Disease Studies

Methylation array studies enable researchers to understand the functional mechanisms at work in complex disease. Illumina offers complementary solutions for researchers studying these disorders. Learn more about complex disease research.

Epigenetics and Cancer
Cancer Epigenetics

Illumina array and sequencing solutions are ideal for detecting altered methylation patterns and epigenetic changes that provide insight into tumorigenic pathways and cancer progression. Learn more about cancer epigenetics.

DNA methylation-based classification of central nervous system tumours.

Nature 555 469-74 2018

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Clinical epigenomics: genome-wide DNA methylation analysis for the diagnosis of Mendelian disorders.

Genetics in Medicine  23 1065-74 2021

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Reprogramming to recover youthful epigenetic information and restore vision.

Nature  2020 588 124-29

Read Article
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Methylation Sequencing
Methylation Sequencing

Methylation sequencing approaches offer higher density and flexibility in assessing epigenome changes.

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FastTrack Microarray Services
FastTrack Microarray Services

Illumina experts provide methylation array and genotyping services.

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Methylation Array Data Analysis
Methylation Array Data Analysis Tips from Experts

Experts describe their approaches to analyzing Illumina methylation array data.

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Epigenome-Wide Association Studies
Epigenome-Wide Association Studies

Epigenetic changes in newborns linked with maternal smoking during pregnancy.

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