Small genome sequencing (≤ 5 Mb) involves sequencing the entire genome of a bacterium, virus, or other microbe, and then comparing the sequence to a known reference. Sequencing small microbial genomes can be useful for food testing in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.
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Unlike traditional approaches, small genome sequencing studies using next-generation sequencing (NGS) do not rely on labor-intensive cloning steps. NGS also enables biologists to sequence hundreds of organisms simultaneously via multiplexing. NGS can identify low-frequency variants, genomic rearrangements, and other genetic changes that might be missed or are too costly to identify using other methods. For small genomes, DNA libraries can be prepared, sequenced, and analyzed in as little as 2 days.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Speed and simplicity for targeted and small genome sequencing applications, processing up to 384 small genomes per run.
Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of global sequencing data.*
In addition to our industry-leading data quality, Illumina offers integrated workflows that simplify small genome sequencing, from library preparation to data analysis.
NGS, by rapidly characterizing the full viral genome, plays a critical role in the outbreak of emerging viruses by enabling phylogenetic characterization without a prior knowledge of the potential family. This detailed viral information is enabling public health officials to respond with unprecedented speed and breadth.Read Article
Learn how Joseph Petrosino, PhD uses whole-genome shotgun sequencing to obtain functional gene pathway and strain-based information in microbiome studies. These methods provide researchers and pharmaceutical companies with data to refine drug discovery and development.Read Article
Microbial whole-genome sequencing is an important research tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. Learn more about microbial whole-genome sequencing.
Shotgun metagenomics enables comprehensive analysis of all genes in all organisms present in a given complex sample. It enables researchers to detect very low abundance microbes that may be missed or are too expensive to identify using other methods. Learn more about shotgun metagenomic sequencing.
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*Data calculations on file. Illumina, Inc., 2015