NGS Library Preparation

Experience Faster Library Prep

Easy adoption with superior support across the entire workflow. Fast, simple library prep and enrichment workflow from Illumina.

  • DNA
  • RNA
  • Enrichment
  • Amplicon
  • Epigenetics

The foundation to discovery and insights. Quality, precision, and ease of use in every step with our library prep portfolio advancements.

Get more out of your samples, reduce hands-on time, and save money. Benefit from the best customer support using the entire Illumina workflow.

See What’s Possible for Your Experiments*


*Specifications for Illumina DNA Prep kit. This is just one example; see chart below for specifications on other time-saving library prep kits.

Do More with Illumina Library Prep

NGS library preparation with continual innovation. The result: a workflow that is even easier to use, scalable for any size lab, requires a small number of steps, and has a fast workflow time. The library preparation process involves converting a genomic DNA sample (or cDNA sample) into a library of fragments which can then be sequenced on an NGS instrument. Breakthrough technology in our library prep helps you get to answers in less time.

Breadth of NGS Library Prep Lets You Ask Virtually Any Question

Illumina library prep protocols accommodate a range of throughput needs, from lower-throughput protocols for small labs to fully automated library preparation workstations for large laboratories or genome centers. Our solutions support a broad range of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.

Illumina Library Prep: Designed for a Perfect Fit

Not only do our library preparation solutions save you time and money, they are optimized specifically for our instruments and secondary analysis platform, DRAGEN. Even those new to NGS will find the workflows and applications are easy to adopt. In addition, you’ll experience superior support across the entire workflow because our customer support team will have more information to help you troubleshoot your sequencing runs.

Fast, simple library prep from Illumina

Illumina DNA library prep kits and RNA library prep kits enable rapid generation of high quality samples and sequencing data. Optimized for Illumina sequencing platforms, each kit provides highly reproducible results across sample types and experiments—even from the most challenging samples. Watch the video and discover the ease of adoption with superior support across the entire workflow.

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Fast, simple library prep from Illumina

Related Solutions


Our partners have developed both high- and low-throughput walk-away automation methods that span our library prep portfolio.

Learn More
Index Adapters

Increase the number of samples sequenced per run and optimize high-throughput sequencing using unique dual index adapters.

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Unique molecular identifiers (UMIs) provide error correction and accuracy. They can reduce false-positive variant calls while increasing variant detection sensitivity.

Learn More
Complete Library Preparation in an Afternoon

Learn about technology advancements and innovations that provide you with novel, easy-to-use, and fast solutions for both DNA and RNA library preparation.

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Workflow time and automatability brings significant advantages in increasing workflow efficiency

Lab Director, Reference Lab


Application Whole-genome sequencing Whole-genome sequencing DNA enrichment - no UMI
Hands-on time ~45 min 1-1.5 hrs ~2 hrs
Turnaround time ~1.5 hrs ~3-4 hrs ~6.5 hrs
Input 25 ng to 300 ng 1 ng to 500 ng 10 ng to 1000 ng
Automation available Yes Yes Yes
PCR protocol No Yes Yes
Library Quant needed? No No No
Fragmentation included? Yes – on bead Yes – on bead Yes – on bead
Product Illumina DNA PCR-Free Prep Illumina DNA Prep Illumina DNA Prep with Enrichment
Application Whole transcriptome mRNA RNA enrichment
Hands-on time < 3 hrs < 3 hrs < 2 hrs
Turnaround time ~7 hrs 6.5 hrs < 9 hrs
Input 1 to 1000 ng standard quality RNA; 10 ng for optimal performance and FFPE samples 25 to 1000 ng standard quality RNA 10 ng standard quality RNA; 20 ng RNA for low quality / degraded / FFPE
Automation capability Liquid handling robots Liquid handling robots Liquid handling robots
PCR protocol No No Yes
Library Quant needed? Yes Yes Yes
Fragmentation included? Yes Yes Not required
Product Illumina Stranded Total RNA Prep Illumina Stranded mRNA Prep Illumina RNA Prep with Enrichment

Ask an expert how you can get the most with Illumina Library Prep

We'll walk through your needs and make recommendations.

Innovation that continues to raise the bar and optimize the workflow

Key NGS Library Preparation Technologies

Our innovative NGS library preparation portfolio uses three key technologies. Find out the benefits of each approach and how they differ.

Bead-Linked Transposome Tagmentation

This technology uses bead-bound transposomes for a more uniform reaction compared to in-solution tagmentation reactions.

Adapter Ligation

The ligation process prepares NGS libraries by fragmenting a genomic DNA or cDNA sample and ligating specialized adapters to both fragment ends.

Amplicon Library Prep

With a PCR-based workflow and ease of use for users new to NGS, amplicon library prep can measure thousands of targets simultaneously.

Library Prep Technical Tips

DNA/RNA Isolation Considerations

Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.

dsDNA Library Concentration

Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.

Quantification and Quality Control

Find out how to quantify and validate final libraries for a successful sequencing run.

Library Normalization Best Practices

Learn when library normalization is required, and how to perform the necessary steps.

What is the PhiX Control v3 Library?

Learn how to use the PhiX library as a control in your Illumina sequencing runs.

Nucleic Acid Quality Assessment

Simultaneous qualification and quantification of nucleic acids with the Fragment Analyzer.