Comprehensive NGS library preparation solutions

NGS Library Preparation

Library preparation involves generating a collection of DNA fragments for sequencing. Next-generation sequencing (NGS) libraries are typically prepared by fragmenting a genomic DNA sample and ligating specialized adapters to both fragment ends. An alternative process called "tagmentation" combines the fragmentation and ligation reactions into a single step. Adapter-ligated fragments are then PCR amplified and gel purified.

What is NGS Library Preparation

A key element of Illumina NGS is high-quality library preparation. Illumina library prep protocols can accommodate a range of throughput needs, from lower-throughput protocols for small laboratories to fully automated library preparation workstations for large laboratories or genome centers.

Illumina offers comprehensive sequencing library preparation solutions for a wide variety of NGS methods, including whole-genome sequencing, targeted DNA sequencing, whole-transcriptome sequencing, targeted RNA sequencing, and more. These solutions support a variety of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.

Illumina offers an extensive assortment of easy-to-use next-generation sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. To find the right kit for your needs, use the selection tool below, or view a filterable list of library prep kits.

Library Prep Kit Selector Library Prep Kit Selector
Kits for Preparing NGS Libraries

For labs preparing large quantities of NGS libraries, liquid-handling robots and additional automation solutions provide high-throughput capacity and important quality control touch points. Visit the page below to learn more about Illumina-qualified sequencing library prep automation methods available through our partners.

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Automated Library Prep Buyer's Guide

This guide will walk you through the range of automation tools that exist for NGS library preparation, and help you find the right solution for your needs.

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NGS Workflow Steps

Learn the basics of each step in the Illumina sequencing workflow and find out how to prepare before starting the library preparation process.

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Featured Microbial Whole-Genome Sequencing Workflow
Quantification and Quality Control

Find out how to quantify and validate final libraries for a successful sequencing run.

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DNA/RNA Isolation Considerations

Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.

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dsDNA Library Concentration

Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.

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Sequencing Library Preparation Methods

Access a summary of NGS methods compiled from peer-reviewed publications. Explore the wide range of questions that can be addressed with Illumina technology. Find method descriptions as well as pros and cons.

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Methods Guide

All the information you need, from BeadChips to library preparation to sequencer selection and analysis. Select the best tools for your lab.

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