Library preparation involves generating a collection of DNA fragments for sequencing. Next-generation sequencing (NGS) libraries are typically prepared by fragmenting a genomic DNA sample and ligating specialized adapters to both fragment ends. An alternative process called "tagmentation" combines the fragmentation and ligation reactions into a single step. Adapter-ligated fragments are then PCR amplified and gel purified.
A key element of Illumina NGS is high-quality library preparation. Illumina library prep protocols can accommodate a range of throughput needs, from lower-throughput protocols for small laboratories to fully automated library preparation workstations for large laboratories or genome centers.
Illumina offers comprehensive sequencing library preparation solutions for a wide variety of NGS methods, including whole-genome sequencing, targeted DNA sequencing, whole-transcriptome sequencing, targeted RNA sequencing, and more. These solutions support a variety of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.
Illumina offers an extensive assortment of easy-to-use next-generation sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. To find the right kit for your needs, use the selection tool below, or view a filterable list of library prep kits.
For labs preparing large quantities of NGS libraries, liquid-handling robots and additional automation solutions provide high-throughput capacity and important quality control touch points. Visit the page below to learn more about Illumina-qualified sequencing library prep automation methods available through our partners.See Automation Options
Find out how to quantify and validate final libraries for a successful sequencing run.Read Technical Bulletin
Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.Read Technical Bulletin
Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.Read Technical Bulletin
Access a summary of NGS methods compiled from peer-reviewed publications. Explore the wide range of questions that can be addressed with Illumina technology. Find method descriptions as well as pros and cons.