Flexibility for multiple sequencing applications

The NextSeq 550 System enables a wide range of applications in a benchtop sequencer

NextSeq Applications & Methods

mRNA sequencing (mRNA-Seq) enables researchers to take a closer look at gene expression and can be used to identify isoforms, novel transcripts, and gene fusions. The NextSeq 550 RNA sequencing solution leverages proven Illumina technology to deliver a detailed snapshot of the coding transcriptome. Sequence up to 16 transcriptomes per NextSeq 550 run.
Exome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. Exome sequencing with the NextSeq 550 System offers a simple and reliable method to identify coding variants, successfully sequencing even the most difficult genomic regions. Sequence 3–12 exomes per NextSeq run.

See how researchers use the NextSeq 550 System for exome sequencing applications.

Get a detailed genome view of the smallest organisms. Small genome sequencing provides comprehensive analysis of microbial or viral genomes for public health, epidemiology, and disease studies. Sequence up to 384 small genomes per NextSeq 550 run.

Featured Stories

 
NGS Identifies Rare Disease Variants in Infants with Undiagnosed Disease
NGS Identifies Rare Disease Variants in Infants with Undiagnosed Disease

Landmark NGS study leads to a national clinical diagnostic exome sequencing platform in Singapore.

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Uncovering the Intracellular Communication of Tumors
Uncovering the Intracellular Communication of Tumors

The microenvironment surrounding tumors could tell us more about how cancer cells migrate and become drug resitant.

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Microarray Scanning

In addition to sequencing, the NextSeq 550 enables microarray scanning for a broad range of applications in reproductive health, genetic health, and cancer research, including methylation analysis with the Infinium MethylationEPIC BeadChip.

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Target Enrichment

Use sequence-specific hybridization to analyze genomic regions of interest.

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Whole-Transcriptome Sequencing

Analyze both coding RNA and multiple forms of noncoding RNA with total RNA sequencing, for a comprehensive view of the transcriptome.

Application Note

NextSeq 550 System RNA-Seq Solution

Researcher Interview

Stromal Signatures May Inform Cancer Prognosis

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Coding Transcriptome Analysis

Detect both known and novel features of the RNA exome using sequence-specific capture of RNA coding regions.

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Targeted DNA Resequencing

Targeted resequencing focuses time, expenses, and analysis on sequencing only a subset of genes or genome regions of research interest.

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Unmasking the Viral Etiology of Cancer and Immune Disease

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Targeted Gene Panels

Targeted gene sequencing panels contain defined probe sets focused on specific genes of interest. Both predesigned and custom panels are available.

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Methylation Sequencing

Perform epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.

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Amplicon Sequencing

Ultra-deep sequencing of PCR amplicons enables cost-effective analysis of up to hundreds of target genomic regions in one assay.

Application Note

NextSeq 550 System Amplicon Sequencing Solution

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De Novo Sequencing

De novo sequencing with next-generation sequencing (NGS) enables fast, accurate characterization of species without a reference genome.

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Delivering Sequencing Results Quickly and at a Competitive Price

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Genotyping by Sequencing

Genotyping by sequencing provides a low-cost genetic screening method to discover novel plant and animal SNPs and perform genotyping studies.

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DNA-Protein Interaction Analysis with ChIP-Seq

Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.

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NGS Proves Invaluable for Biomarker Discovery

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miRNA & Small RNA Analysis

Isolate and sequence small RNA species, such as microRNA, to study the role of noncoding RNA in gene silencing and posttranscriptional regulation.

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Unraveling Disease Mechanisms With the NextSeq 500 System

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Translation Analysis

Ribosome profiling enables systematic monitoring of cellular translation processes and prediction of protein abundance.

Application Note

Ribosome Profiling Enables Comprehensive Translation Analysis

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Casting a Genome-Wide Net Over Malaria

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Metagenomics

Comprehensively sample genes in organisms present in a complex sample to evaluate bacterial diversity and detect unculturable microorganisms.

Application Note

Metagenomic Analysis of Environmental Water Samples

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Cytogenomic Microarrays and Sequencing

Next-generation sequencing offers base-by-base, genome-wide detection of chromosomal variation to complement array-based cytogenomic analysis.

Researcher Interview

From Arrays to NGS: Evolution of Chromosomal Genetics

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RNA Drug Response Biomarker Discovery

Find out how to utilize RNA-Seq to discover and profile RNA-based drug response biomarkers. Access resources designed to help new users adopt this application.

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Methylation Arrays

Quantitatively interrogate methylation sites across the genome at single-nucleotide resolution.

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Illumina Methods Guide

Methods Guide

Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.

Access Guide