MiniSeq System Reagent kits have a ready-to-use cartridge with two-channel SBS chemistry for cost-efficient sequencing, even for low sample numbers.
MiniSeq Reagent Kits bring the power and reliability of proven Illumina sequencing by synthesis (SBS) technology to labs of all sizes at an affordable price without sacrificing data quality. These kits offer:
The MiniSeq Reagent Kits offer multiple options for both sequencing output and read length configurations to support a wide range of applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. In addition, innovations to the MiniSeq Rapid Reagent Kit enable faster run times for NGS workflows to improve lab efficiency and provide a quicker time to results, compared to MiniSeq standard reagents. MiniSeq Rapid reagents reduce sequencing run times to < 5 hours, enabling faster detection of respiratory viruses and pathogens, including SARS-CoV-2.
MiniSeq Reagent Kit |
No. of Reads |
Kit Size (cycles) |
Output (max.) |
2 × 150 Output |
2 × 75 Output |
1 × 75 Output |
| MiniSeq High Output Kit | 25 M | 75, 150, 300 |
7.5 Gb |
7.5 Gb |
3.75 Gb |
1.875 Gb |
| MiniSeq Mid Output Kit |
8 M |
300 |
2.4 Gb |
2.4 Gb |
1.2 Gb |
600 Mb |
| MiniSeq Rapid Kit | 20 M |
100 |
2 Gb |
N/A |
N/A |
N/A |
Gb = gigabases, M = millions
| Instruments | MiniSeq System |
|---|---|
| Maximum output | 7.5 Gb (300-cycle high output kit), 3.75 Gb (150-cycle high output kit), 2.4 Gb (300-cycle mid output kit), 1.875 Gb (75-cycle high output kit), 2.0Gb (100-cycle Rapid kit) |
| Maximum reads per run | Up to 25 million (high output kits), Up to 8 million (mid output kits), Up to 20 million (Rapid kit) |
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Sequencing control, Cluster generation, Sequencing by synthesis |
| Technology | Sequencing |
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Sequencing coverage calculator
Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.
As a hypothesis-free method, NGS can distinguish between infectious disease strains that differ by as little as one SNP, and replace multiple tests.
Illumina sequencing allows researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism.
MiniSeq Rapid Reagent Kit (100 cycles)
20044338
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 100-cycle single read, rapid run on the MiniSeq System.
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MiniSeq High Output Reagent Kit (75-cycles)
FC-420-1001
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 75-cycle High Output run on the MiniSeq System.
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MiniSeq High Output Reagent Kit (150-cycles)
FC-420-1002
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 150-cycle High Output run on the MiniSeq System.
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MiniSeq High Output Reagent Kit (300-cycles)
FC-420-1003
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 25M reads). Includes: High Output Reagent Cartridge (300 cycles), Flow Cell, and Hybridization Buffer.
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MiniSeq Mid Output Kit (300-cycles)
FC-420-1004
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 8M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Flow Cell, and Hybridization Buffer.
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Order a MiniSeq System today. Speak to a representative for information on pricing, availability, and more.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
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