Understanding the role of methylation in regulating gene expression

DNA Methylation Analysis

DNA methylation analysis can help scientists gain valuable insight into gene regulation and identify potential biomarkers. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction. DNA methylation is also a common subject of agrigenomic investigations into responses to drought, temperature extremes, and other environmental changes.

High-throughput technologies such as next-generation sequencing (NGS) and microarrays enable genome-wide methylation profiling studies. These technologies offer new ways to understand the significance of DNA methylation, providing novel insights into the functional consequences of epigenetic variation.

Methylation Sequencing with NGS

NGS enables comprehensive profiling of methylation patterns at single-base resolution across the whole genome, or in targeted epigenetic regions of interest.

Methylation Microarrays

Arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.

Most important to me Comprehensive methylome coverage High throughput (large sample numbers)
Least important to me Throughput Coverage
#CpGs covered

~36 million CpGs (whole genome)

~3.3. million CpGs (targeted)

3,000 – 850,000 CpGs

All (whole genome)

Human (targeted)

Human & Mouse (flagship arrays)

All others (custom arrays)

  Learn More About Methylation Sequencing Learn More About Methylation Arrays
Field Guide to Methylation Methods

An overview of the impact of cytosine methylation and the primary methods used to detect cytosine modifications.

Access PDF
Field Guide to Methylation Methods
Methylome and Clinical Outcomes

Genetic epidemiologist Dr. Kathleen C. Barnes discusses how methylation arrays add another dimension to the study of COVID-19 and other infectious diseases.

Read Interview
Epigenetic Contributions to Addiction

Targeted methylation sequencing identifies epigenetic signatures that correlate with brain image scans of substance abusers.

Read Interview
Methylation and Metastasis

Researchers investigate the epigenetics behind cancer metastasis using DNA methylation microarrays and NGS.

Read Interview
Epigenetic Changes in Cancer
Epigenetic Changes in Cancer

Studies of epigenetic alterations in cancer, such as aberrant methylation and transcription factor binding, can provide insight into important tumorigenic pathways. Learn more about cancer epigenetics research.

Gene Target ID for Complex Disease Research
Gene Target Identifcation in Complex Diseases

Combining methylation analysis and differential gene expression analysis can help scientists prioritize genes for further study and elucidate complex disease mechanisms. Learn more about gene target ID.

Chromatin Accessibility Analysis
Chromatin Accessibility Analysisr

ATAC-Seq does not require prior knowledge of regulatory elements, making it a powerful epigenetic discovery tool. It is a popular method for determining chromatin accessibility across the genome. Learn more about ATAC-Seq.

Genome-Wide Association Studies (GWAS)
Genome-Wide Association Studies (GWAS)

Genome-wide association studies (GWAS) use high-throughput genomic techniques to quickly scan entire genomes of large groups to find genetic variants correlated with a trait or disease. Learn more about GWAS.

Interested in receiving newsletters, case studies, and information on genomic analysis techniques? Enter your email address.
When Whole-Genome Sequencing Is Not Enough
When Whole-Genome Sequencing Is Not Enough

Researchers sequence whole genomes, exomes, and epigenomes to explore cancer subtypes.

View Video
Epigenome Roadmap
Epigenome Roadmap

A collection of research papers that systematically characterize epigenomic landscapes in primary human tissues and cells.

Access Roadmap
Epigenome Blueprint
Epigenome Blueprint

A consortium generating 100 reference epigenomes to understand gene activation and repression in healthy and diseased human cells.

Learn More
DNA Methylation Analysis
TruSeq Methyl Capture EPIC

Rapidly produce target enrichment-based bisulfite sequencing libraries from human DNA samples.

View Product