Illumina Complete Long Read Prep, Human

Illumina Complete Long Read Prep, Human is available for order through your sales rep. Contact your Illumina sales representative to order now.

Illumina Complete Long Read Prep, Human

Highly accurate, high-performance full workflow solution for comprehensive human WGS with long-read data from NovaSeq platforms. Read More...

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Illumina Complete Long Read Prep, Human (24 samples)

20089108

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Illumina Complete Long Read Prep, Human (8 samples)

20086823

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llumina Unique Dual Indexes, LT (48 Indexes, 48 Samples)

20098166

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Illumina Complete Long Read DRAGEN Cloud Analysis

20100421

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Illumina Complete Long Read Prep, Human - 24, S4 starter pack

20099754

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Illumina Complete Long Read Prep, Human - 24, 10B starter pack

20099755

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Product Highlights

With Illumina Complete Long Reads technology, long-read sequencing is now accessible and streamlined for genomic labs. Illumina Complete Long Read Prep, Human is the first product to utilize this technology, providing the most comprehensive view of the genome. The high-performance whole-genome sequencing assay uses a standard NGS workflow to generate contiguous long-read sequences on the NovaSeq 6000 System and NovaSeq X Series.

Streamlined, accessible assay with long and short reads on a single platform, compatible wth the NovaSeq X Series and NovaSeq 6000 System
Optimized standard NGS library prep and DRAGEN secondary analysis for highly accurate, reliable results
Robust and flexible performance with low input requirements, no specialized extraction, shearing, or size selection required
Efficient, single-day, automation-friendly workflow that is more scalable than on-market long-read solutions

Highly accurate, scalable assay

Illumina Complete Long Read data complements standard short-read WGS data and delivers more comprehensive whole genomes by:

Calling variants in challenging, difficult-to-map regions with high homology or repetitive regions
Resolving complex structural variants, pseudogenes, and large insertion–deletions (indels)
Phasing variants and calling haplotypes

This novel technology will bright more light to even the darkest corners of the genome. Illumina Complete Long Reads helps resolve the most challenging regions of the genome and makes long-read sequencing accessible and streamlined by enabling short and long reads from a single platform.

Introducing Illumina Complete Long Read Sequencing Technology

Frequently Purchased Together

Specifications

Project Recommendations

Instrument	Recommended Number of Samples	Read Length
NovaSeq 6000 System	4 samples (1 sample per lane of an S4 flow cell). 8 samples (1 sample per lane of an S4 flow cell, dual flow cell run optional). A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended). NovaSeq 6000 Xp Workflow kit.	2 × 150 bp
NovaSeq X System	4 samples (1 sample per 2 lanes of a 10B flow cell). A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended).	2 × 150 bp
NovaSeq X Plus System	8 samples (1 sample per 2 lanes of a 10B flow cell, dual flow cell run). A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended).	2 × 150 bp

Method-Specific Workflow Example

Library Preparation

Illumina Complete Long Read Prep Recommended: Illumina DNA PCR-Free Prep

Sequencing*

NovaSeq 6000 System

NovaSeq 6000Dx System (RUO mode)

NovaSeq X Series

Data Analysis

DRAGEN Illumina Complete Long Read app on BaseSpace Sequence Hub

How the Illumina Complete Long Read technology works

Tagmentation is used to normalize long fragment sizes. Long fragments are "land-marked" to capture single-molecule, long-read information and amplified. Marked fragments are tagmented to standard libraries for sequencing. Marked and unmarked data are combined to generate highly accurate complete long reads. Learn more about how Illumina Complete Long Read technology works.

How the Illumina Complete Long Read technology works

Long-read phased sequencing enables clear haplotype assignment

Illumina Complete Long Reads can resolve highly polymorphic regions like the HLA-A gene. Uniform coverage in the HLA region enables accurate phasing of HLA alleles.

Supporting Data and Figures

Long-read sequencing can detect large deletions

A heterozygous 180 bp deletion in the SEMG1 gene is clearly detected by both Illumina Complete Long Reads and on-market long reads.

Supporting Data and Figures

Long-read sequencing can resolve pseudogenes

Both Illumina Complete Long Reads and on-market long reads clearly resolve the 23 kb STRC gene from its pseudogene, pSTRC.

Supporting Data and Figures

Long-read sequencing can identify STR insertions

Insertions in short tandem repeat (STR) regions are clearly detected by both Illumina Complete Long Reads and on-market long reads.

Supporting Data and Figures

Long-read sequencing can resolve pseudogenes

Both Illumina Complete Long Reads and on-market long reads clearly resolve the SULT1A1 gene (a pharmacogenomic marker) from its pseudogene, SULT1A2.

Supporting Data and Figures

High-quality performance across a wide range of DNA input

Illumina Complete Long Read Prep, Human with DNA inputs from 5 ng to 1200 ng (in triplicate) generates similar data quality metrics for N50 and phase block N50. N50 is defined as the sequence length of the shortest contig (or phase block) at 50% of the total assembly length.

Supporting Data and Figures

In this presentation, we share use cases of Complete Long Reads and highlight research being done by collaborators around the world.

Watch on-demand webinar

Illumina Complete Long-Read technology webinar

Illumina Complete Long Read technology will accelerate access to the remaining ~5% of genic regions. Hear Andrew Shaver, Product Manager describe the benefits of the Illumina novel long-read technology, including the accuracy, scalability, and workflow benefits. See how the Illumina Complete Long Read technology complements the technical innovations in our Illumina product portfolio.

Watch on-demand webinar

Illumina Complete Long-Read technology: Detailed insights into complex genomes

Product Literature

Technical Note | PDF | 3 versions

Data Sheet | PDF | 8 versions

Manuals and Support Information

Illumina Complete Long Read Prep Product Documentation

DRAGEN ICLR WGS (Illumina Complete Long Reads) Online Help Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All Illumina Complete Long Reads Support

Illumina DNA PCR-Free Prep

Illumina DNA PCR-Free Prep

A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.

NovaSeq 6000 Reagent Kits

NovaSeq 6000 Reagent Kits

Reagent kits for the NovaSeq 6000 system provide ready-to-use cartridge-based reagents for cluster generation and SBS.

NovaSeq X Series Reagent Kits

NovaSeq X Series Reagent Kits

Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.

Interested in the Illumina Complete Long Read Prep, Human Kit?

Speak with one of our specialists about how your workflow and analysis can benefit from the accuracy and scalability of our long-read technology. Fill out the form to request a quote for ordering today.

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Rare Disease Whole-Genome Sequencing

Whole-genome sequencing is the most comprehensive test for rare disease, with the potential for superior diagnostics and outcomes.

Learn More

Whole-Genome Sequencing (WGS)

Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.

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Genetic & Rare Diseases

NGS technology is helping to drive breakthroughs in genetic disease testing by facilitating early detection and diagnosis.

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* Sequencing Illumina Complete Long Read libraries on NovaSeq platforms may cause the reported Q30 score of a run to fall below the NovaSeq specification. This does not indicate a performance issue with the sequencing run, nor the library.