Illumina Complete Long Read Prep, Human is available for order through your sales rep. Contact your Illumina sales representative to order now.
Illumina Complete Long Read Prep, Human
Highly accurate, high-performance full workflow solution for comprehensive human WGS with long-read data from NovaSeq platforms.
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Product Highlights
With Illumina Complete Long Reads technology, long-read sequencing is now accessible and streamlined for genomic labs. Illumina Complete Long Read Prep, Human is the first product to utilize this technology, providing the most comprehensive view of the genome. The high-performance whole-genome sequencing assay uses a standard NGS workflow to generate contiguous long-read sequences on the NovaSeq 6000 System and NovaSeq X Series.
- Streamlined, accessible assay with long and short reads on a single platform, compatible wth the NovaSeq X Series and NovaSeq 6000 System
- Optimized standard NGS library prep and DRAGEN secondary analysis for highly accurate, reliable results
- Robust and flexible performance with low input requirements, no specialized extraction, shearing, or size selection required
- Efficient, single-day, automation-friendly workflow that is more scalable than on-market long-read solutions
Highly accurate, scalable assay
Illumina Complete Long Read data complements standard short-read WGS data and delivers more comprehensive whole genomes by:
- Calling variants in challenging, difficult-to-map regions with high homology or repetitive regions
- Resolving complex structural variants, pseudogenes, and large insertion–deletions (indels)
- Phasing variants and calling haplotypes
Introducing Illumina Complete Long Read Sequencing Technology
This novel technology will bright more light to even the darkest corners of the genome. Illumina Complete Long Reads helps resolve the most challenging regions of the genome and makes long-read sequencing accessible and streamlined by enabling short and long reads from a single platform.
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Frequently Purchased Together
Specifications
Project Recommendations
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NovaSeq 6000 System | 4 samples (1 sample per lane of an S4 flow cell). 8 samples (1 sample per lane of an S4 flow cell, dual flow cell run optional). A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended). NovaSeq 6000 Xp Workflow kit. |
2 × 150 bp |
| NovaSeq X System | 4 samples (1 sample per 2 lanes of a 10B flow cell). A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended). |
2 × 150 bp |
| NovaSeq X Plus System | 8 samples (1 sample per 2 lanes of a 10B flow cell, dual flow cell run). A paired PCR-free WGS library is required for analysis (Illumina DNA PCR-Free Prep recommended). |
2 × 150 bp |
How the Illumina Complete Long Read technology works
Tagmentation is used to normalize long fragment sizes. Long fragments are "land-marked" to capture single-molecule, long-read information and amplified. Marked fragments are tagmented to standard libraries for sequencing. Marked and unmarked data are combined to generate highly accurate complete long reads. Learn more about how Illumina Complete Long Read technology works.
Long-read phased sequencing enables clear haplotype assignment
Illumina Complete Long Reads can resolve highly polymorphic regions like the HLA-A gene. Uniform coverage in the HLA region enables accurate phasing of HLA alleles.
Long-read sequencing can detect large deletions
A heterozygous 180 bp deletion in the SEMG1 gene is clearly detected by both Illumina Complete Long Reads and on-market long reads.
Long-read sequencing can resolve pseudogenes
Both Illumina Complete Long Reads and on-market long reads clearly resolve the 23 kb STRC gene from its pseudogene, pSTRC.
Long-read sequencing can identify STR insertions
Insertions in short tandem repeat (STR) regions are clearly detected by both Illumina Complete Long Reads and on-market long reads.
Long-read sequencing can resolve pseudogenes
Both Illumina Complete Long Reads and on-market long reads clearly resolve the SULT1A1 gene (a pharmacogenomic marker) from its pseudogene, SULT1A2.
High-quality performance across a wide range of DNA input
Illumina Complete Long Read Prep, Human with DNA inputs from 5 ng to 1200 ng (in triplicate) generates similar data quality metrics for N50 and phase block N50. N50 is defined as the sequence length of the shortest contig (or phase block) at 50% of the total assembly length.
Illumina Complete Long Reads webinar
In this presentation, we share use cases of Complete Long Reads and highlight research being done by collaborators around the world.
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Illumina Complete Long Read technology: Detailed insights into complex genomes
Illumina Complete Long Read technology will accelerate access to the remaining ~5% of genic regions. Hear Andrew Shaver, Product Manager describe the benefits of the Illumina novel long-read technology, including the accuracy, scalability, and workflow benefits. See how the Illumina Complete Long Read technology complements the technical innovations in our Illumina product portfolio.
Watch on-demand webinar
Product Literature
Technical Note | PDF | 8 versions
Data Sheet | PDF | 8 versions
Manuals and Support Information
Illumina Complete Long Read Prep Product Documentation
DRAGEN ICLR WGS (Illumina Complete Long Reads) Online Help Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
NovaSeq 6000 Reagent Kits
Reagent kits for the NovaSeq 6000 system provide ready-to-use cartridge-based reagents for cluster generation and SBS.
NovaSeq X Series Reagent Kits
Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.
Interested in the Illumina Complete Long Read Prep, Human Kit?
Speak with one of our specialists about how your workflow and analysis can benefit from the accuracy and scalability of our long-read technology. Fill out the form to request a quote for ordering today.
* Sequencing Illumina Complete Long Read libraries on NovaSeq platforms may cause the reported Q30 score of a run to fall below the NovaSeq specification. This does not indicate a performance issue with the sequencing run, nor the library.