Whole-genome sequencing (WGS) is a comprehensive method for analyzing entire genomes. Genomic information has been instrumental in identifying inherited disorders, characterizing the mutations that drive cancer progression, and tracking disease outbreaks. Rapidly dropping sequencing costs and the ability to produce large volumes of data with today’s sequencers make whole-genome sequencing a powerful tool for genomics research.
While this method is commonly associated with sequencing human genomes, the scalable, flexible nature of next-generation sequencing (NGS) technology makes it equally useful for sequencing any species, such as agriculturally important livestock, plants, or disease-related microbes.
Unlike focused approaches such as exome sequencing or targeted resequencing, which analyze a limited portion of the genome, whole-genome sequencing delivers a comprehensive view of the entire genome. It is ideal for discovery applications, such as identifying causative variants and novel genome assembly.
Whole-genome sequencing can detect single nucleotide variants, insertions/deletions, copy number changes, and large structural variants. Due to recent technological innovations, the latest genome sequencers can perform whole-genome sequencing more efficiently than ever.
Illumina Distinguished Scientist Gary Schroth discusses the latest advances in Illumina whole-genome sequencing solutions and the vast potential of emerging genomics technologies.Access Webinar
Sequencing large genomes (> 5 Mb), such as human, plant, or animal genomes, can provide valuable information for disease research and population genetics.
Small genome sequencing (≤ 5 Mb) involves sequencing the entire genome of a bacterium, virus, or other microbe. Without requiring bacterial culture, researchers can sequence thousands of small organisms in parallel using NGS.
De novo sequencing refers to sequencing a novel genome where there is no reference sequence available. NGS enables fast, accurate characterization of any species.
Phased sequencing, or genome phasing, distinguishes between alleles on homologous chromosomes, resulting in whole-genome haplotypes. This information is often important for genetic disease studies.
Previously a challenging application, human whole-genome sequencing has never been simpler. It offers the most detailed view into our genetic code.
Long reads can help resolve challenging regions of the genome, such as those containing highly variable or highly repetitive elements.
This user-friendly three-step WGS workflow provides a fully featured, rapid solution for labs and delivers high-quality insights across the entire genome for all variant classes.
Illumina is providing whole-genome sequencing for a UK-wide study led by Genomics England, designed to compare the genomes of severely and mildly ill COVID-19 patients.
Whole-genome shotgun sequencing and transcriptomics provide researchers and pharmaceutical companies with data to refine drug discovery and development.
A New England Journal of Medicine study found that using WGS to assess leukemia samples produced more accurate results, in less time, than karyotyping or fluorescence in situ hybridization.
Illumina Distinguished Scientist Gary Schroth discusses the latest WGS advances and the vast potential of emerging genomics technologies.
Watch how improvements in informatics are advancing whole-genome sequencing, and find out the latest on Illumina Complete Long Reads.
Compare sequencing platforms by application and specification. Find tools and guides to help you choose the right instrument.
WGS offers a promising option for building accurate drug-metabolizing enzyme allele frequency databases for pharmacogenomics.
Determine the best kit for your needs based on your project type, starting material, and method of interest.
Find high-quality whole-genome and other sequencing services that deliver analyzed data to researchers.
Use this interactive tool to explore experimental NGS library preparation methods compiled from the scientific literature.
This novel technology will bright more light to even the darkest corners of the genome. Illumina Complete Long Reads helps resolve the most challenging regions of the genome and makes long-read sequencing accessible and streamlined by enabling short and long reads from a single platform.Learn about the technology and products
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This library is derived from the small, well-characterized bacteriophage genome, PhiX. It is an ideal sequencing control for run quality monitoring.
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